摘要
目的观察脯氨酰寡肽酶(POP)抑制剂S17092对脂质超载人肝细胞株LO2脂质沉积的改善作用,并探讨其机制。方法将LO2细胞随机分为3组,对照组LO2细胞用1%无脂肪酸(FFA)的1640培养基培养,模型组LO2细胞用FFA制备脂质超载模型后再用1640培养基培养,实验组LO2细胞制备脂质超载模型后再加入含S17092的1640培养液培养。各组细胞继续培养24 h后,采用油红O染色法观察各组细胞内脂质沉积情况,采用Bio-Rad蛋白质测定法检测各组细胞内甘油三酯(TG),采用实时荧光定量PCR法检测各组细胞内POP、固醇调节元件结合蛋白-1c(SREBP-1c)、脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶1(ACC1)、硬脂酰辅酶A去饱和酶(SCD1)mRNA。结果油红O染色结果显示,与对照组相比,模型组LO2细胞内含有大量的红染颗粒,而实验组较模型组红染颗粒相对较少。对照组、模型组、实验组细胞内TG水平分别为(1.995±0.221)pmole/μL、(2.910±0.030)pmole/μL、(2.573±0.113)pmole/μL,组间相比,P均<0.05。与对照组相比,模型组、实验组细胞中POP、SREBP-1c、FAS、ACC1、SCD1 mRNA相对表达量明显升高(P均<0.05);与模型组相比,实验组细胞中POP、SREBP-1c、FAS、ACC1、SCD1 mRNA相对表达量明显降低(P均<0.05)。结论POP抑制剂S17092可改善脂质超载LO2细胞的脂质沉积,其机制可能与抑制SREBP-1c及其靶基因的表达有关。
Objective To observe the improved effect of prolyl oligopeptidase(POP)inhibitors(S17092)on lipid deposition of lipid overloaded human liver cell line LO2,and to explore its mechanism.Methods LO2 cells were ran-domly divided into three groups.LO2 cells in the control group were cultured in 1640 culture medium with 1%free fatty ac-id(FFA).LO2 cells in the model group were cultured in 1640 culture medium after preparation of lipid overload model with FFA,and LO2 cells in the experimental group were cultured in 1640 culture solution containing S17092 after prepar-ing the lipid overload model.After 24 h of continuous culture,lipid depositions of cells in each group were observed by oil red O staining,and intracellular triglyceride(TG)in each group was detected by Bio-Rad protein assay.The levels of in-tracellular prolyl oligopeptidase(POP),sterol regulatory element binding protein-1c(SREBP-1c),fatty acid synthase(FAS),acetyl coenzyme A carboxylase-1(ACC1)and stearyl coenzyme A desaturase(SCD1)mRNA in each group were detected by real-time quantitative PCR.Results The results of oil red O staining showed that compared with the control group,there were a large number of red stained particles in the LO2 cells of the model group.The red stained particles in the experimental group were less than those of the model group.The levels of intracellular TG in control group,model group and experimental group were(1.995±0.221)pmole/μL,(2.910±0.030)pmole/μL and(2.573±0.113)pmole/μL respectively,P<0.05.Compared with the control group,the mRNA relative expression levels of POP,SREBP-1c,FAS,ACC1 and SCD1 in model group and experimental group were significantly increased(all P<0.05).Compared with the model group,the mRNA relative expression of POP,SREBP-1c,FAS,ACC1 and SCD1 in experimen-tal group were significantly decreased(all P<0.05).Conclusion POP inhibitor S17092 can improve lipid deposition in lipid-overloaded LO2 cells,and the mechanism may be related to the inhibition of SREBP-1c and its target genes expres-sion.
作者
舍玲
丁永年
纪文静
阿孜古力·阿不来提
SHE Ling;DING Yongnian;JI Wenjing;Aziguli·Abulaiti(Department of Digestive Medicine,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)
出处
《山东医药》
CAS
2021年第21期9-12,共4页
Shandong Medical Journal
基金
新疆维吾尔自治区自然科学基金资助项目(2019D01C230)。
