摘要
[目的]探索出一套红花香椿无性快繁技术方法。[方法]以红花香椿组织培养的方式进行无性繁殖育苗,测定不同阶段红花香椿生长情况。[结果]诱导阶段当3/4MS+6-BA0.2、NAA0.1时,新芽的比例较高,是较适宜的诱导培养基;继代阶段1/2DCR+6-BA 0.2 mg/L'+NAA 0.04 mg/L使丛生芽的增殖倍数最高;生根阶段培养基采用改良MS+IBA 1.0 mg/L+NAA 0.05 mg/L生根率最为理想;温室中炼苗时间为1周后移栽成活率较高。[结论]该研究为建立红花香椿茎段诱导及植株再生体系提供参考。
[Objective]To explore a set of asexual rapid propagation technology of Toona rubriflora Tseng.[Method]The asexual propagation of Toona rubriflora Tseng was carried out by tissue culture.The growth of Toona rubriflora Tseng in different stages was determined.[Result]The results showed that,when 3/4MS+6-BA0.2、NAA0.1 were used in induction stage the proportion of new shoots was higher.It was the most suitable induction medium with the subculture stage,1/2DCR+6-BA 0.2 mg/L'+NAA 0.04 mg/L+NAA 0.04 mg/l made the multiplication multiple of clustered buds the highest.The best rooting rate was MS+IBA 1.0 mg/L+NAA 0.05 mg/L in the rooting stage medium.The survival rate of transplanting was higher after one week in greenhouse.[Conclusion]This study provided a reference for the establishment of stem segment induction and plant regeneration system of Toona rubriflora Tseng.
作者
郑雪燕
李勇
郑天汉
彭王勋
郑宏
ZHEN Xue-yan(Fujian Yangkou State-owned Forest Farm,Shunchang,Fujian 353200)
出处
《园艺与种苗》
CAS
2021年第7期1-2,6,共3页
Horticulture & Seed
基金
福建省科技厅农业引导性(重点)项目“红花香椿优良种质选育及开发利用研究”(闽科计[2018]8号)。
关键词
红花香椿
芽器官
离体培养
快速繁殖
Toona rubriflora Tseng
Bud organ
In vitro culture
Rapid propagation
