摘要
为准确评价雪茄烟种质资源的遗传多样性,利用SSR引物对92份可用作雪茄烟原料的种质进行分析,共检测到234个等位基因,平均为5.4419,每个位点的多态性信息量变化范围为0.0995~0.8469,平均值为0.6082;Nei’s多样性指数平均为0.6546,Shannon信息指数平均达1.3035。UPGMA聚类分析在遗传距离0.35处可将供试种质分为4个类群,PCA主成分分析将供试材料划分为4个类群,Structure群体遗传结构分析将供试材料划分为5个类群;不同分析方法划分的类群虽然有所差异,但分析结果均与地理来源有一定的相关性。从中筛选出15对核心引物构建了供试种质的指纹图谱,为建立雪茄烟种质资源鉴定体系奠定理论基础。本研究可为雪茄烟鉴定评价、遗传背景研究和种质创新应用等提供科学依据。
In order to evaluate the genetic diversity of cigar germplasm resources accurately,92 cigars were analyzed with SSR primers.A total of 234 alleles were amplified and the average number was 5.4419.The polymorphic information content of each locus varied from 0.0995 to 0.8469 with an average of 0.6082.The average diversity index of Nei was 0.6546,and the average of Shannon information index was 1.3035.Unweighted pair-group method with arithmetic means cluster analysis showed that the tested cigar resources can be divided into 4 groups at the genetic distance of 0.35.The test materials were divided into 4 groups by principal component analysis and 5 groups by structure population genetic structure analysis.Although there were some differences in the groups divided by different analysis,the results were all related to the geographical origin.15 core-primers were screened to construct the fingerprint database of the tested germplasms,which laid a theoretical foundation for the establishment on the identification system of cigar germplasm resources.This study provided scientific reference for cigar identification and evaluation,genetic background research,germplasm innovation and application.
作者
向小华
刘国祥
张兴伟
夏长剑
李媛
陈荣平
杨菁
赵文涛
吴达
李方友
吕洪坤
XIANG Xiaohua;LIU Guoxiang;ZHANG Xingwei;XIA Changjian;LI Yuan;CHEN Rongping;YANG Jing;ZHAO Wentao;WU Da;LI Fangyou;LYU Hongkun(Haikou Cigar Research Institute,Hainan Provincial Branch of China National Tobacco Corporation,Haikou 571100,China;Tobacco Research Institute,Chinese Academy of Agricultural Science,Qingdao 266101,China;Shanghai New Tobacco Product Research Institute Limited Company,Shanghai 200082,China;Shanghai Tobacco Group Co.,Ltd,Shanghai 200082,China)
出处
《中国烟草科学》
CSCD
北大核心
2021年第4期9-16,共8页
Chinese Tobacco Science
基金
中国烟草总公司海南省公司项目(201846000024055、2020460000270099)。
