摘要
目的探讨阿司匹林抵抗相关基因血小板内皮聚集受体1(PEAR1)和前列腺素内过氧化物合酶1(PTGS1)多态性与缺血性脑卒中神经功能缺损程度的相关性。方法收集2018年10月至2019年12月盐城市第一人民医院收治的缺血性脑卒中病人147例,根据美国国立卫生研究院卒中量表(NIHSS)评分将其分为轻症组和中重症组,应用聚合酶链反应(PCR)-荧光法进行PEAR1和PTGS1基因多态性检测,利用多因素回归分析阿司匹林相关基因多态性与缺血性脑卒中神经功能缺损的关系。结果两组病人年龄[(63.7±12.8)岁比(68.6±10.7)岁]、吸烟[18.46%(12/65)比35.37%(29/82)]、糖尿病患病率[24.62%(16/65)比42.68%(35/82)]、空腹血糖[(5.87±1.69)mmol/L比(7.38±3.11)mmol/L]、D-二聚体[0.31(0.19,0.61)mg/L比0.55(0.26,1.12)mg/L]差异有统计学意义(P<0.05);两组病人PEAR1基因型分布差异有统计学意义(P<0.05),而PTGS1基因型分布差异无统计学意义(P>0.05);通过logistic回归分析发现,年龄(OR:1.047,95%CI:1.023-1.072;P<0.001)、吸烟(OR:2.876,95%CI:1.548-5.343;P=0.001)、空腹血糖(OR:1.390,95%CI:1.193-1.621;P<0.001)、PEAR1基因携带A等位基因(OR:1.786,95%CI:1.028-3.101;P=0.040)与缺血性脑卒中神经功能缺损严重程度相关(P<0.05)。结论缺血性脑卒中神经功能缺损严重程度与年龄、吸烟、空腹血糖、PEAR1基因携带A等位基因有一定的相关性。
Objective To explore the relevance between aspirin-resistance-related gene polymorphisms of platelet endothelial aggregation receptor 1(PEAR1)and prostaglandin-endoperoxide synthase 1(PTGS1)and neurological impairment in patients with ischemic stroke.Methods The medical records of 147 patients with ischemic stroke admitted to The First People’s Hospital of Yancheng from October 2018 to December 2019 were collected.According to NIHSS score,the patients were assigned into mild group and medium to severe group.The genotypes of PEAR 1 and PTGS1 were detected by polymerase chain reaction(PCR)-fluorescence technique.Multivariate regression analysis was used to analyze the correlation between aspirin-resistance-related gene polymorphism and neurological impairment in patients with ischemic stroke.Results There were significant differences in age[(63.7±12.8)years vs.(68.6±10.7)years],smoking[18.46%(12/65)vs.35.37%(29/82)],prevalence of diabetes mellitus[24.62%(16/65)vs.42.68%(35/82)],fasting blood glucose[(5.87±1.69)mmol/L vs.(7.38±3.11)mmol/L]and D-dimer[0.31(0.19,0.61)mg/L vs.0.55(0.26,1.12)mg/L]between the two groups(P<0.05).There was significant difference in PEAR1 genotype distribution between the two groups(P<0.05),while no significant difference in PTGS1 genotype distribution(P>0.05).Logistic regression analysis showed that age(OR:1.047,95%CI:1.023-1.072;P<0.001),smoking(OR:2.876,95%CI:1.548-5.343;P=0.001),fasting blood glucose(OR:1.390,95%CI:1.193-1.621;P<0.001)and A allele of PEAR1 gene(OR:1.786,95%CI:1.028-3.101;P=0.040)were correlated with the severity of neurological impairment in patients with ischemic stroke(P<0.05).Conclusion The severity of neurological impairment in patients with ischemic stroke may be associated with age,smoking,fasting blood glucose and A allele of PEAR1 gene.
作者
吴慧
缪阳
陈迎平
祁峰
卞海林
WU Hui;MIAO Yang;CHEN Yingping;QI Feng;BIAN Hailin(Pharmaceutical Department,The First People’s Hospitalof Yancheng,Yancheng,Jiangsu 224000,China)
出处
《安徽医药》
CAS
2021年第10期2004-2008,共5页
Anhui Medical and Pharmaceutical Journal
