摘要
目的研究神经胶质瘤患者血清中CD26水平的表达,并探讨其临床意义。方法根据2016版WHO中枢神经系统肿瘤分类收集郑州大学附属肿瘤医院住院的70例不同病理级别的胶质瘤患者血液样本,应用酶联免疫吸附试验(ELISA)测定70例患者手术前和20例健康对照组血清CD26蛋白水平变化,并分析与WHO病理分级及患者临床病理特征的相关性。应用实时荧光定量PCR(qRT-PCR)技术检测不同病理级别胶质瘤组患者和对照组CD26 mRNA水平变化。应用在线String数据库、GO功能注释和京都基因与基因组百科全书KEGG数据库分析CD26相互作用的可能相关蛋白PPI网络及参与的信号通路。结果胶质瘤患者手术前血液中CD26蛋白水平和mRNA水平显著高于健康对照组(P=0.000,P<0.01),并且随着WHO病理分级的增高而增加(P=0.000)。进一步研究发现胶质瘤患者血清CD26水平与IDH1/2的状态密切相关(P<0.05),与年龄、性别、原发肿瘤直径、部位、远处转移、ATRX状态、放化疗以及癫痫发生病史无关(P>0.05)。PPI网络结果发现与CD26蛋白相互作用的10个蛋白分子网络。GO功能注释和KEGG通路富集分析显示CD26参与遗传发育、信号通路过程。结论CD26水平在胶质瘤中显著增加并与胶质瘤的病理级别密切相关,可作为胶质瘤的新型分子标志物,有助于评估病情和评价疗效。
Objective To explore the changes of CD26 level in patients with different grades of glioma and its clinical significance.Methods The serum of 70 patients with different pathological grades were collected in the affiliated Cancer Hospital of Zhengzhou University according to the 2016 WHO classification of central nervous system tumors.The serum CD26 levels of 70 patients and 20 healthy controls were measured by ELISA.Furthermore,CD26 mRNA level was tested using qRT-PCR method.The relationship between CD26 level and age,sex and diameter of primary tumor were investigated.Online String database,GO functional annotation and KEGG database were used to analyze the PPI network and signal pathway of CD26 interaction.Results The mRNA and protein level of CD26 in patients with glioma before operation was significantly higher than that in healthy controls(P=0.000),companied by increasing pathological grades(P=0.000,P<0.01).Furthermore,it was found that the level of serum CD26 in patients with glioma was closely related to the status of IDH1/2(P<0.05),but not related to age,sex,diameter of primary tumor,location,distant metastasis,ATRX status,radiotherapy and chemotherapy and history of epilepsy(P>0.05).PPI network results showed that 10 protein networks interacted with CD26 protein.GO functional annotation and KEGG pathway enrichment analysis showed that CD26 was involved in genetic development and signal pathway.Conclusion Serum CD26 can be used as a molecular marker of glioma.Detection of serum CD26 level before and after operation is helpful to evaluate the condition and curative effect of glioma.
作者
张玉松
韩亚迪
冯稳
Zhang Yusong;Han Yadi;Feng Wen(Affiliated Cancer Hospital of Zhengzhou University,Henan 450008,China)
出处
《医学研究杂志》
2021年第9期68-71,158,共5页
Journal of Medical Research
基金
河南省医学科技攻关计划项目(2018020500)。
