摘要
本研究选用叶锈菌生理小种260,与小麦近等基因系Tc Lr26及其轮回亲本Thatcher (Tc)分别组成不亲和组合和亲和组合,在7日龄小麦叶片上接种叶锈菌,采用实时定量PCR(RT-qPCR)对TaSnRK1基因的表达进行分析。并通过构建pSuper1300+-SnRK1表达载体对TaSnRK1进行亚细胞定位分析。由RT-qPCR结果可知,该基因在不亲和组合中,表达量随时间延长先升高后下降,且在12 h达到峰值;而在亲和组合中,表达量也呈现先升高后下降趋势,但表达量远远低于不亲和组合。亚细胞定位结果表明,TaSnRK1蛋白分布在细胞质和细胞核中。本研究结果为进一步深入探讨TaSnRK1的功能和作用机制奠定了基础。
In this study, the physiological race 260 of Puccinia triticina was selected to form incompatible combinations and compatible combinations with the near-isogenic line Tc Lr26 of wheat and its recurrent parent Thatcher(Tc). Seven-day-old wheat leaves were inoculated with Puccinia triticina and real-time quantitative(RTqPCR) was used to analyze the expression of TaSnRK1 gene. By constructing pSuper1300+-SnRK1 expression vector, we analyzed the subcellular location of TaSnRK1. From the RT-qPCR results, we found that in the incompatibility interaction combinations, the expression level of TaSnRK1 increased at first and then decreased with time, and reached the peak after 12 hours. In the compatible combinations, the expression level also showed the same trend, but the expression level was much lower. The subcellular localization results showed that TaSnRK1 protein was distributed in the cytoplasm and nucleus. This experiment lays a foundation for the further study of the function and mechanism of TaSnRK1.
作者
汤文倩
王冬梅
TANG Wenqian;WANG Dongmei(State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/College of Life Sciences,Hebei Agricultural University,Baoding 071001,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2021年第4期7-12,共6页
Journal of Hebei Agricultural University
基金
国家自然科学基金(31171472、31871548)
高等学校博士学科点专项科研基金资助课题(20111302130001)
河北省应用基础研究计划重点基础研究项目(12967149D)
2020年度创新能力提升计划外专引才引智专项-河北省引进国外智力项目。
关键词
小麦
叶锈菌
SnRK1
亚细胞定位
wheat
Puccinia triticina
SnRK1
subcellular localization
