摘要
为了筛选口蹄疫(foot-and-mouth disease,FMD)疫苗的新型多糖佐剂,选用新疆荒漠肉苁蓉粗多糖与口蹄疫灭活抗原(inactivated foot-and-mouth disease viral antigen,FMDV-Ag)制备成水佐剂疫苗,ISA-206油佐剂疫苗和无佐剂疫苗,通过肌肉途径免疫ICR小鼠,检测免疫后血清抗体水平、淋巴细胞增殖反应、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)和CD4^(+)CD44^(+)、CD8^(+)CD44^(+)T细胞亚群的表达水平等评价疫苗的免疫增强效果,并且监测小鼠免疫后的行为和体重变化观察安全性。肉苁蓉粗多糖可以显著提高FMDV特异性的抗体水平(P<0.05);同时显著增强了FMDV特异性的淋巴细胞增殖水平(P<0.05),显著促进了CD4^(+)、CD8^(+)和CD44^(+)T细胞比例(P<0.05)。免疫后小鼠没有观察到异常行为,且对小鼠体重增长没有影响。肉苁蓉粗多糖作为FMDV-Ag的水佐剂通过增强体液和细胞免疫水平发挥良好的免疫促进作用。
To screen new polysaccharide vaccine adjuvants of foot-and-mouth disease,the crude polysaccharide from Cistanche deserticola in Xinjiang(CDCP)and inactivated foot-and-mouth disease viral antigen(FMDV-Ag)were selected to prepare water adjuvant vaccines,ISA-206 oil adjuvant vaccine and adjuvant-free vaccine.ICR mice were immunized by muscle.Serum antibody level,lymphocyte proliferation response,and expressions of CD3^(+)CD4^(+),CD3^(+)CD8^(+)and CD4^(+)CD44^(+),CD8^(+)CD44^(+)T cell subsets were measured to evaluate the immune enhancement effect after the vaccination,and the behaviors and weights of the mice were monitored for observing the safety of the vaccines.The results showed CDCP substantially enhanced the FMDV-specific antibody level(P<0.05)and significantly increased lymphocyte proliferative responses(P<0.05).T responses were also significantly enhanced(P<0.05),including proliferative responses of CD4^(+),CD8^(+),and CD44^(+)T cells.No abnormal behavior for mice after immunization was observed and the weights of mice also were not affected.CDCP as water adjuvant of FMDV-Ag has a fine immune-promoting effect by enhancing humoral and cellular immune response.
作者
张爱莲
巴雪丽
王丹阳
赵兵
ZHANG Ai-lian;BA Xue-li;WANG Dan-yang;ZHAO Bing(College of Life Science and Technology,Xinjiang University,Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,Urumqi 830046)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第9期212-218,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(31660259,31960164)。
关键词
新疆荒漠肉苁蓉
粗多糖
口蹄疫疫苗
佐剂
Cistanche deserticola in Xinjiang
crude polysaccharide
foot-and-mouth disease viral vaccine
adjuvant
