摘要
Achieving real-time,continuous and long-term monitoring of dopamine(DA)in vivo is essential for revealing brain functions and preventing and treating neurogenic diseases.However,it remains challenging to achieve a low limit of detection(LOD)and high neuron-compatibility at the same time for the current microsensors,resulting in the failure of long-term and accurate detection of DA in vivo.A DA-sensing fiber was achieved by the potentiometric method to possess a low LOD of 5 nM,1-3 orders of magnitude lower than amperometry and differential-pulse voltammetry.The sensing fiber showed a wide linear range from 5 to 185 nM that well matched the DA concentration(26-40 nM)in vivo.After implantation,the sensing fiber showed no influence on the firing rates of neurons with the potentiometric test,indicating high neuron-compatibility.It was then integrated with electrophysiology to simultaneously monitor DA variation and electrical signal in the brain,with stable monitoring of DA change in vivo for 8 weeks.The sensing fiber was flexible and stably worked after hundreds of bending,and it showed high sensitivity even after protein adsorption,thus offering a reliable tool for neuroscience.
基金
the National Natural Science Foundation of China(22075050,21634003)
the Ministry of Science and Technology of China(2016YFA0203302)
the Science and Technology Commission of Shanghai Municipality(20JC1414902)
Shanghai Municipal Education Commission(2017-01-07-00-07-E00062)。
