摘要
目的探讨临床病理技术在胸腔积液细胞块病理组织学诊断中的应用价值。方法选取2020年1—12月医院收治的64例胸腔积液患者作为研究对象,采集患者胸腔积液标本,离心留取沉渣,分别制成细胞涂片和细胞块切片,均行HE染色和免疫组化染色,统计两种检查方法对胸腔积液的定性诊断结果,并以最终病理诊断结果为金标准评价定性诊断准确率,且观察两种检查方法的免疫标记染色结果。结果64例患者,经最终病理诊断结果显示,良性33例,恶性31例。细胞块切片免疫组化染色的诊断准确率高于细胞涂片HE染色及免疫组化染色与细胞块切片HE染色,差异有统计学意义(P<0.05);细胞涂片免疫组化染色的诊断准确率高于细胞涂片HE染色,差异有统计学意义(P<0.05);细胞块切片免疫组化染色中细胞角蛋白17(CK17)、癌胚抗原(CEA)、上皮细胞表面糖蛋白-31(MOC-31)的阳性检出率均高于细胞涂片,差异有统计学意义(P<0.05)。结论临床病理技术是定性诊断胸腔积液的有效方法,其中以细胞块切片免疫组化染色诊断的准确率最高,可为胸腔积液定性诊断及治疗提供参考。
Objective The application value of clinicopathological techniques in the histopathological diagnosis of pleural effusion cell mass was explored.Methods 64 patients with pleural effusion admitted to the hospital from January to December in 2020 were taken as the research objects.The patients'pleural effusion samples were collected,centrifuged to collect the sediment,and then made into cell smears and cell block sections.HE staining and immunohistochemical staining were carried out.The qualitative diagnosis results of pleural effusion by the two inspection methods were analyzed,and taken the final pathological diagnosis results as the gold standard,the accuracy of the qualitative diagnosis was evaluated.Besides,the immunolabeling staining results of the two inspection methods were observed.Results The final pathological diagnosis results showed that among 64 patients,33 cases were benign and 31 cases were malignant.The accuracy of immunohistochemical staining of cell block sections in the qualitative diagnosis of pleural effusion was higher than that of HE staining&immunohistochemical staining of cell smears and HE staining of cell block sections,and the difference was statistically significant(P<0.05).The accuracy of immunolabeling staining of cell smears was higher than that of HE staining of cell smears,and the difference was statistically significant(P<0.05);The positive rate of cytokeratin 17(CK17),carcino-embryonic antigen(CEA)and epithelial cell surface glycoprotein-31(MOC-31)of immunohistochemical staining of cell block sections were all higher than those of cell smears,and the difference was statistically significant(P<0.05).Conclusions Clinicopathological technique is an effective method for the qualitative diagnosis of pleural effusion.Among them,the diagnostic accuracy of immunohistochemical staining on the section of cell block is the highest,which could provide references for the qualitative diagnosis and treatment of pleural effusion.
作者
张真
Zhang Zhen(Department of Pathology,The First Affiliated Hospital of Fujian Medical University,Fuzhou Fujian 350004,China)
出处
《医疗装备》
2021年第19期63-65,共3页
Medical Equipment
关键词
胸腔积液
细胞块
免疫组化染色
诊断价值
Pleural effusion
Cell block
Immunohistochemical staining
Diagnostic value
