摘要
甘油-3-磷酸酰基转移酶(glycerol-3-phosphate acyltransferase,GPAT)是三酰甘油(triacylglycerol,TAG)生物合成的限速酶,催化TAG生物合成的起始步骤,为多种脂质合成提供了底物,会直接参与到植物的生长发育和抗逆过程。蒺藜苜蓿(Medicago truncatula)作为豆科模式植物具有基因组小、生长周期短、遗传转化效率高等特点。为了解GPAT基因在苜蓿抗逆尤其是在耐盐中的作用,本研究选择蒺藜苜蓿基因组为研究对象,采用Blastp和Hmm结构域搜索方法,共鉴定出24个mtGPAT基因。根据系统进化、基因结构和结构域差异将其分成3个亚家族。同时染色体定位分析发现,24个mtGPAT基因不均匀分布在7条苜蓿染色体上,每条染色体上分布有2~5个基因。基因表达谱分析表明:蒺藜苜蓿GPAT基因具有器官特异性,并且会参与盐胁迫反应。这些结果可为进一步深入研究蒺藜苜蓿GPAT家族基因的功能提供理论基础。
Glycerol-3-phosphate acyltransferase(GPAT)is a rate-limiting enzyme in the biosynthesis of triacylglycerol(TAG).GPAT catalyzes the initial step of TAG biosynthesis,provides substrates for synthesis of a variety of lipids,and is directly involved in plant growth,plant development,and stress resistance.Medicago truncatula,a leguminous model plant,has the characteristics of a small genome,short growth cycle,and high genetic transformation efficiency.Thus,the genome of M.truncatula was selected as the research object in this study to understand the role of the GPAT gene in alfalfa stress resistance,especially in salt tolerance.Twenty-four mtGPAT genes were identified from the genome of M.truncatula by BLASTp and HMM domain searches.According to the phylogenetic analysis,gene structure,and domain differences,the genes can be divided into three subfamilies.Chromosome mapping analysis showed that 24 mtGPAT genes were unevenly distributed on 7 alfalfa chromosomes,and 2~5 genes were distributed on each chromosome.Finally,gene expression pattern analysis showed that the GPAT gene of M.truncatula was organ-specific and involved in salt stress response.These results can provide a theoretical basis for further study of the function of GPAT family genes in M.truncatula.
作者
杨成兰
段瑞君
武雄雄
祁存英
马银花
熊辉岩
YANG Chenglan;DUAN Ruijun;WU Xiongxiong;QI Cunying;MA Yinhua;XIONG Huiyan(College of Eco-Environmental Engineering,Qinghai University,Xining 810016,Qinghai,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China)
出处
《草业科学》
CAS
CSCD
北大核心
2021年第10期1966-1974,共9页
Pratacultural Science
基金
国家自然科学基金(31560052)
青海省科技厅应用基础研究项目(2019-ZJ-7049)。
