摘要
MYB家族已被证实在植物生长发育、类黄酮合成、环境胁迫、木质素生物合成等多个生理方面发挥着重要的作用。为探究阳桃MYB家族在木质素生物合成过程中的作用,本研究基于全基因组数据,对阳桃(Averrhoa carambola L.)R2R3-MYB转录因子进行了全面的生物信息学分析,并采用RT-qPCR技术,对8个阳桃R2R3-MYB基因在阳桃小枝3个发肓时期(T1、T3、T6)、果和叶的表达情况进行了验证。结果显示,阳桃基因组中共包含57个1R-MYB、100个R2R3-MYB、4个3R-MYB,100个阳桃R2R3-MYB(AcMYBs)基因不均匀地分布在11条染色体上,被分为24个亚组,相同亚组的基因具有相似但不完全相同的基因结构和保守基序。片段重复和串联重复在阳桃MYB家族的扩张中发挥了重要的作用,且重复产生的基因都经历了纯化选择。同时,阳桃R2R3-MYB家族与拟南芥(Arabidopsis thaliana(L.)Heynh.)及毛果杨(Populus trichocarpa Torr.&A.Gray)都具有较强的共线性关系。RT-qPCR结果表明,除AcMYB05外,AcMYB41、AcMYB65、AcMYB84、AcMYB87、AcMYB92、AcMYB97和AcMYB100都在T3和T6时期呈现出较高的表达,在T1和果、叶中几乎不表达。本研究表明,7个AcMYBs的表达具有明显组织特异性,其可能参与了阳桃木质素生物合成,为进一步研究MYB家族对木质素生物合成的影响提供了重要的参考价值。
MYB family transcription factors have been proved to play an important role in biological process,such as plant growth and development,flavonoid synthesis,environmental stress,lignin biosynthesis.To explore if the MYB family members are involved in the lignin biosynthesis in Averrhoa carambola L.,a genome-wide bioinformatics analysis of R2R3-MYB transcription factor has been conducted,followed by the RT-qPCR analysis to valid the expression levels of the eight R2R3-MYB genes at different stages in twigs(T1,T3,T6),fruits and leaves.Fifty-seven 1R-MYB,100 R2R3-MYB and four 3R-MYB have been identified.Out of them,100 R2R3-MYB(AcMYBs)were detected to be unevenly distributed on 11 chromosomes and they were divided into 24 subclades(A1-A24;the genes from the same subclade present similarity on gene structures and functional motifs).The family gene expansion might associated to the segmental and tandem duplications,and the duplicated genes were undergone purifying selection.The sequence homology of R2R3-MYB gene family was observed in A.carambola L.,Arabidopsis thaliana(L.)Heynh.and Populus trichocarpa Torr.&A.Gray.RT-qPCR showed that except for AcMYB05,the genes AcMYB41,AcMYB65,AcMYB84,AcMYB87,AcMYB92,AcMYB97 and AcMYB100 were found with higher expression in T3 and T6 and rare transcripts in T1,fruit and leaves.Collectively,the transcriptional profiles suggested the tissue-specific expression of seven AcMYBs,which possibly participate in the lignin biosynthesis pathway of A.carambola L.,therefore providing reference for further analyzing the MYB family members that interplay with the lignin biosynthesis.
作者
赵亚梅
陈蕾
吴春梅
秦思
翟俊文
任惠
吴沙沙
ZHAO Ya-mei;CHEN Lei;WU Chun-mei;QIN Si;ZHAI Jun-wen;REN Hui;WU Sha-sha(College of Landscape Architecture,Fujian Agriculture and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,Fuzhou 350002;Horticulture Research Insistute of Guangxi Academy of Agricultural Science,Nanning 530007)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2021年第6期1746-1761,共16页
Journal of Plant Genetic Resources
基金
广西科技重大专项(桂科AA17204045-4)
广西农业科学院基本科研业务专项(桂农科2021YT046)
福建农林大学艺术学院、园林学院(合署)学科专业建设项目(YSYL-bdpy-1)。
关键词
阳桃
木质素
R2R3-MYB转录因子
全基因组鉴定
表达模式
Averrhoa carambola L.
lignin
R2R3-MYB transcription factors
genome-wide identification
expression pattern analysis
