摘要
建立了超高效液相色谱法同时测定绿茶茶汤中表没食子儿茶素没食子酸酯、没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、没食子酸、儿茶素没食子酸酯、表儿茶素、表没食子儿茶素、儿茶素、没食子儿茶素、茶氨酸、咖啡因、可可碱等12种有效成分的方法。称取茶叶样品粉末1.00 g,用100 mL 90℃的水溶解,并于90℃水浴中超声提取20 min,加水定容至100 mL,取1 mL样品溶液,用水定容至10 mL,离心,上清液用0.22μm滤膜过滤。以Waters ACQUITY UPLC BEH C_(18)色谱柱为固定相,以不同体积比的0.01%(体积分数)磷酸溶液和乙腈的混合液为流动相进行梯度洗脱,用二极管阵列检测器在210 nm处对样品进行扫描。结果显示:12种有效成分在16 min内便可分离,12种有效成分的质量浓度在一定范围内与其对应的峰面积呈线性关系,检出限(3S/N)为3.2~15.6 mg·kg^(-1),保留时间和峰面积的相对标准偏差(n=6)分别为0.13%~0.23%和0.56%~3.9%。对实际样品进行加标回收试验,回收率为89.2%~113%。
A method for simultaneous determination of 12 effective ingredients,including gallic catechin gallic acid ester,gallnut catechin gallic acid ester,epicatechin gallic acid ester,gallic acid,catechins gallic acid ester,epicatechin,table gallic catechin,catechin,gallic catechin,theanine,caffeine and theobromine in green tea soup by ultra-high performance liquid chromatography(UHPLC)was established.Tea powder sample of 1.00 g was dissolved with 100 mL of 90℃water and extracted by ultrasound in 90℃water bath for 20 min,then water was used to make its volume up to 100 mL.The above sample solution of 1 mL was taken and made its volume up to 10 mL with water.After centrifuge,the supematant was filtered by 0.22μm filter membrane.Waters ACQUITY UPLC BEH C_(18) column was used as stationary phase and a mixture of 0.01%(φ)phosphoric acid solution and acetonitrile with different volume ratios were used as mobile phase for gradient elution.The sample was detected by diode array detector at detection wavelength of 210 nm.The results showed that 12 effective ingredients could be separated within 16 min.Linear relationships between mass concentrations of 12 effective ingredients and their peak areas were kept in the definite range,with detection limits(3 S/N)in the range of 3.2-15.6 mg·kg^(-1).RSDs(n=6)of retention times and peak areas were found in the ranges of 0.13%-0.23%and 0.56%-3.9%,respectively.Recovery test was made on actual samples by standard addition method,giving results in the range of 89.2%-113%.
作者
胡毅
王微
兰吉玉
鄢人雨
刘文锋
HU Yi;WANG Wei;LAN Jiyu;YAN Renyu;LIU Wenfeng(Guizhou University,Guiyang 550025,China;Qiandongnan Testing Center for Agricultral Products Quality and Safety,Kaili 556000,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2021年第10期903-907,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
超高效液相色谱法
儿茶素类
茶氨酸
生物碱
ultra-high performance liquid chromatography
catechin
theanine
alkaloid
