摘要
为了探究东北红豆杉野生种和栽培种之间代谢物质的差异,对其进行非靶向代谢组学的分析。利用GC-MS技术以及多元统计分析对野生种和栽培种的树皮进行比较,发现两者代谢物质有差异,总共检测到45种显著差异代谢物,主要包括4种氨基酸、10种有机酸和一些糖醇等。其中有22个代谢物质表达上调,23个表达下调,通过KEGG进行通路分析发现其中有7条通路影响较显著,分别为硫代谢,精氨酸和脯氨酸代谢,磷酸戊糖途径,不饱和脂肪酸的生物合成,半乳糖代谢,丙氨酸、天冬氨酸和谷氨酸代谢,苯丙烷类生物合成。其中检测到的代谢物如氨基酸和有机酸在栽培种中有更高的表达,东北红豆杉野生种在抗逆性能上弱于栽培种。而糖类却在野生种中表达更高,东北红豆杉野生种拥有更好的糖代谢能力,来抵御低温胁迫,本实验为指导东北红豆杉野生种的保护和东北红豆杉的抗逆性机理的研究提供了理论依据。
In order to explore the differences of metabolites between wild and cultivated Taxus cuspidata,nontargeted metabolomics was analyzed.Using GC-MS and multivariate statistical analysis to compare the barkof wild species and cultivated species,the differences in their metabolites were found.A total of 45 significantmetabolites were detected,including 4 amino acids,10 organic acids and some sugar alcohols.Among them,22 metabolites were up-regulated and the 23 metabolites were down-regulated.KEGG pathway analysis showedthat seven pathways had significant effects,including sulfur metabolism,arginine and proline metabolism,pentose phosphate pathway,unsaturated fatty acid biosynthesis,galactose metabolism,alanine,aspartate andglutamic acid metabolism,phenylpropanoid biosynthesis.The metabolites such as amino acids and organicacids were highly expressed in cultivated T.cuspidata.The stress resistance of wild T.cuspidata was weakerthan that of cultivated T.cuspidata.However,the expression of carbohydrate was higher in wild species.T.cuspidata wild species had better glucose metabolism ability to resist low temperature stress.The resultsprovided a theoretical basis for the protection of T.cuspidata wild species and stress resistance mechanism of T.cuspidata.
作者
刘龙桀
吴可心
刁云飞
李中跃
穆立蔷
LIU Long-Jie;WU Ke-Xin;DIAO Yun-Fei;LI Zhong-Yue;MU Li-Qiang(Forestry College,Northeast Forestry University,Harbin 150040;Mudingjiang Forest Ecosystem Research Station,Harbin 150081)
出处
《植物研究》
CAS
CSCD
北大核心
2021年第5期798-806,共9页
Bulletin of Botanical Research
基金
国家科技基础资源调查专项(2019FY100500)
东北红豆杉野外种群的更新扩散机制及保护策略(20189037)。
