摘要
目的分析急性呼吸道感染患儿肠道病毒流行病学特点,建立一种快速、灵敏及特异的肠道病毒VP1基因分型巢式RT-PCR检测方法。方法选取2015年1月-2018年12月该院肠道病毒通用引物阳性的640例急性呼吸道感染患儿,应用巢式RT-PCR进行肠道病毒分型,PCR阳性产物测序分析鉴定,明确肠道病毒流行病学特征和主要基因型。结果 640例阳性样本中,313例测序成功,其中EV71型男性检出率为5.75%(18/313),女性检出率为3.51%(11/313);CA16型男性检出率为8.63%(27/313),女性检出率为7.67%(24/313);CA6型男性检出率为17.89%(56/313),女性检出率为10.86%(34/313);CA10型男性检出率为9.58%(30/313),女性检出率为11.50%(36/313);CA2型男性检出率为1.92%(6/313),女性检出率为1.92%(6/313);CA4型男性检出率为3.19%(10/313),女性检出率为2.88%(9/313);其他型男性检出率为8.31%(26/313),女性检出率为6.39%(20/313)。不同性别患儿肠道病毒CA6型检出率比较差异有统计学意义(χ^(2)=10.756,P<0.05)。按照年龄分组,萧山地区的儿童呼吸道感染病例以1~岁最常见,占阳性例数的30.67%。测序分型的型别包括EV71型29例(9.26%),CA16型51例(16.29%),CA6型90例(28.75%),CA10型66例(21.08%),CA2型12例(3.83%),CA4型19例(6.07%),CB1型14例(4.47%),CB3型9例(2.87%),CB5型12例(3.83%),Echo11型5例(1.59%),Echo30型6例(1.92%)。结论肠道病毒感染患儿主要临床表现以急性上呼吸道感染为主,其优势基因型为CA6型和CA10型,基于VP1基因分型的巢式RT-PCR具有较好的特异性。
Objective To analyze the epidemiological characteristics of enterovirus in children with acute respiratory infection, establish a rapid, sensitive, and specific nested RT-PCR method for enterovirus VP1 genotyping.Methods From January 2015 to December 2018, 640 children with acute respiratory infection and positive universal primer of enterovirus were selected from Zhejiang Xiaoshan Hospital, nested RT-PCR was used for enterovirus genotyping, PCR positive products were collected for sequencing analysis and identification, the epidemiological characteristics and main genotypes of enterovirus were clarified.Results Among 640 positive cases, 313 cases succeeded in gene sequencing, the detection rates of EV71 type in boys and girls were 5.75%(18/313) and 3.51%(11/313), respectively;the detection rates of CA16 type in boys and girls were 8.63%(27/313) and 7.67%(24/313), respectively;the detection rates of CA6 type in boys and girls were 17.89%(56/313) and 10.86%(34/313), respectively;the detection rates of CA10 type in boys and girls were 9.58%(30/313) and 11.50%(36/313), respectively;the detection rates of CA2 type in boys and girls were 1.92%(6/313) and 1.92%(6/313), respectively;the detection rates of CA4 type in boys and girls were 3.19%(10/313) and 2.88%(9/313), respectively;the detection rates of other types in boys and girls were 8.31%(26/313) and 6.39%(20/313), respectively. There was statistically significant difference in detection rate of CA6 type between boys and girls(χ^(2)=10.756, P<0.05).The children were divided into different groups according to age, respiratory tract infection was common in children more than one year, accounting for 30.67%.The types of gene sequencing included EV71 type(29 cases, 9.26%), CA16 type(51 cases, 16.29%), CA6 type(90 cases, 28.75%), CA10 type(66 cases, 21.08%), CA2 type(12 cases, 3.83%), CA4 type(19 cases, 6.07%), CB1 type(14 cases, 4.47%), CB3 type(9 cases, 2.87%), CB5 type(12 cases, 3.83%), Echo11 type(5 cases, 1.59%), and Echo30 type(6 cases, 1.92%).Conclusion The main clinical manifestation of children with enterovirus infection is acute respiratory infection, the dominant genotypes are CA6 type and CA10 type, the specificity of nested RT-PCR based on VP1 genotyping is good.
作者
顾柳芬
杨爱平
汪敏
杨文娟
GU Liu-Fen;YANG Ai-Ping;WANG Min(Zhejiang Xiaoshan Hospital,Hangzhou,Zhejiang 311201,China)
出处
《中国妇幼保健》
CAS
2021年第22期5289-5293,共5页
Maternal and Child Health Care of China
基金
浙江省杭州市科技计划引导项目(20181228Y86)。
