摘要
目的:探讨中药复方糖肾宝对高糖环境下肾小球内皮细胞迁移功能、Fli-1及炎症因子表达的影响。方法:合成Fli-1-siRNA并转染小鼠肾小球内皮细胞。体外培养小鼠肾小球内皮细胞分为正常组(正常血清)、高糖组(30mmol/L葡萄糖+正常血清)、高糖+糖肾宝血清组(30mmol/L葡萄糖+糖肾宝血清)、Fli-1-siRNA组(30mmol/L葡萄糖+Fli-1-siRNA+正常血清)、Fli-1-siRNA+糖肾宝组(30mmol/L葡萄糖+Fli-1-siRNA+糖肾宝血清)、空转组(30mmol/L葡萄糖+Control-siRNA+正常血清)。实时定量PCR检测Fli-1、MCP-1、ICAM-1 mRNA表达水平;Western blot法检测Fli-1、MCP-1、ICAM-1蛋白表达水平;Transwell检测小鼠肾小球内皮细胞迁移率。结果:与正常组比较,高糖诱导各组内皮细胞迁移率显著下降(P<0.01);与高糖组比较,高糖+糖肾宝组、Fli-1-siRNA组和Fli-1-siRNA+糖肾宝组内皮细胞迁移率显著升高(P<0.01)。与正常组比较,高糖组、高糖+糖肾宝组及空转组Fli-1、MCP-1、ICAM-1 mRNA及蛋白表达水平均显著升高(P<0.01,P<0.05);与高糖组比较,高糖+糖肾宝组、Fli-1-siRNA组和Fli-1-siRNA+糖肾宝组Fli-1、MCP-1、ICAM-1 mRNA及蛋白表达水平显著下降(P<0.05,P<0.01)。结论:中药复方糖肾宝可能通过抑制高糖培养小鼠肾小球内皮细胞Fli-1的表达,减少炎症因子的产生,改善内皮细胞迁移率。
Objective:To investigate the effect of Tangshenbao on the migration function of glomerular endothelial cells,Fli-1 and the expression of inflammatory factors in high glucose environment.Methods:Fli-1-siRNA was synthesized and transfected into mouse glomerular endothelial cells.The cultured mouse glomerular endothelial cells in vitro were divided into:normal group(normal serum),high glucose group(30 mmol/L glucose+normal serum),high glucose+Tangshenbao serum group(30 mmol/L glucose+Tangshenbao serum),Fli-1-siRNA group(30 mmol/L glucose+Fli-1-siRNA+normal serum),Fli-1-siRNA+Tangshenbao group(30 mmol/L glucose+Fli-1-siRNA+Tangshenbao serum),the mock group(30 mmol/L glucose+control siRNA+normal serum).The expression levels of Fli-1,MCP-1 and ICAM-1 mRNA detected by Real-time quantitative PCR,the protein levels of Fli-1,MCP-1 and ICAM-1 were detected by Western blot,and the migration rate of glomerular endothelial cells was detected by Transwell.Results:Compared with the normal group,the cell mobility in each group induced by high glucose decreased(P<0.01).Compared with the high glucose group,the cell mobility increased in the high glucose+Tangshenbao group,Fli-1-siRNA group and Fli-1-siRNA+Tangshenbao group(P<0.01).Compared with the normal group,the expression levels of Fli-1,MCP-1 and ICAM-1 mRNA and protein in the high glucose group,the high glucose+Tangshenbao group and the mock group were significantly increased(P<0.01,P<0.05).Compared with high glucose group,the expression levels of Fli-1,MCP-1 and ICAM-1 mRNA and protein in the high glucose+Tangshenbao group,the Fli-1-siRNA group and the Fli-1-siRNA+Tangshenbao group were significantly lower(P<0.05,P<0.01).Conclusion:Tangshenbao may reduce the production of inflammatory factors and improve the migration rate of endothelial cells by inhibiting the expression of Fli-1 in high glucose culture.
作者
向少伟
黄柳慧
陈宇
胡维
黄露
龙韵
贺西南
许雯雯
XIANG Shao-wei;HUANG Liu-hui;CHEN Yu;HU Wei;HUANG Lu;LONG Yun;HE Xi-nan;XU Wen-wen(Department of Nephrology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China;Guangxi University of Chinese Medicine,Nanning 530001,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2021年第10期5854-5858,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81573906)。
关键词
糖肾宝
糖尿病肾病
肾小球内皮细胞
FLI-1
炎症反应
Tangshenbao
Diabetic nephropathy
Glomerular endothelial cells
Fli-1
Inflammatory response
