摘要
小肠存在于富含自由基的微环境中,并且对各种应激源敏感。白藜芦醇是一种抗衰老、抗氧化、抗炎和胰岛素敏感的天然多酚化合物。本研究主要研究白藜芦醇对肠上皮细胞的保护作用,揭示白藜芦醇对氧化应激所诱导的细胞凋亡的作用机制。用过氧化氢处理IPEC-J2细胞以建立氧化应激细胞模型,评估白藜芦醇对模型的保护作用及作用机制。通过分析发现,过氧化氢显著增加细胞内自由基,产生凋亡小体,导致严重的DNA损伤,并显著降低Bcl-2/Bax的比值,同时增加BAD的表达和Caspase-3的裂解。白藜芦醇能保护IPEC-J2细胞抵抗过氧化氢所诱导的凋亡。阻断Akt或Erk通路减弱了白藜芦醇对氧化应激肠上皮细胞的保护作用。结果表明,白藜芦醇通过Erk1/2和Akt通路发挥对过氧化氢诱导的IPEC-J2细胞凋亡的保护作用。
The intestine exists in a microenvironment rich in free radicals and is sensitive to various stressors.Resveratrol(Rel)is an anti-aging,antioxidant,anti-inflammatory and insulin-sensitive natural polyphenol compound.This experiment is mainly to investigate the protective effects of Rel on intestinal epithelial cells and reveal the mechanisms of Rel on oxidative stress induced cell apoptosis.IPEC-J2 cells were treated with H2O2 to establish oxidative stress cell model to evaluate the protective effect and mechanism of Rel on the model.The result showed that H2O2 significantly increased the intracellular free radicals,promoted the production of apoptotic body,led to serious DNA damage and significantly decreased the Bcl-2/Bax ratio and increased the expression of BAD and cleaved caspase-3.Rel could protected IPEC-J2 cells against H2O2-induced apoptosis.Blockage of Akt or Erk pathway attenuated the effects of Rel on H2O2 induced intestinal epithelial cell oxidative stress.Conclusively,Rel exerts a protective effect on IPEC-J2 cell apoptosis induced by H2O2 through the Erk1/2 and Akt pathways.
作者
刘明明
徐兆云
巩宇红
金鑫鑫
袁渤雨
王玮
LIU Mingming;XU Zhaoyun;GONG Yuhong;JIN Xinxin;YUAN Boyu;WANG Wei(College of Veterinary Medicine,Jilin University,Changchun 130062,China;Special Police Academy of Chinese People's Armed Police Force,Beijing 100000,China;Laboratory Animal Center of Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Pharmacology,College of Basic Medical Science,Jilin University,Changchun 130021,China;College of Animal Science,Zhongkai University of Agriculture and Engineering,Guang-zhou 510225,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第11期2225-2230,2262,共7页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31872442)。
