摘要
祁连山是我国西部重要的生态屏障,拥有大量的草地资源。研究草地土壤细菌群落组成及其与土壤环境因子间的关系,可对维持草地生态及了解祁连山土壤细菌群落组成提供一定的数据基础。采用高通量测序技术检测并分析了祁连山默勒镇3种类型草地(高寒草甸、人工草地、沼泽化草甸)0~10 cm土壤细菌群落结构及多样性,并对上述土壤细菌群落与土壤理化因子的相关性进行了系统的研究。结果表明:1)沼泽化草甸土壤全氮、全磷、有机质碳、含水量和硝态氮显著高于高寒草甸和人工草地(P<0.05)。2)3地共测得有效序列1022446条,以97%的一致性将序列聚类,高寒草甸、人工草地、沼泽化草甸OTU聚类均值分别是4917、5233、5075条。3)Shannon和Simpson多样性指数3地间差异不显著,Chao1指数表现为人工草地>高寒草甸>沼泽化草甸(P<0.05)。4)在门水平上,3地均以变形菌门、酸杆菌门、放线菌门和浮霉菌门为主要类群;在属水平上,表现出不同类型草地细菌富集类型不同。5)有机质碳、全磷、土壤含水量与土壤细菌群落的组成表现出极显著相关(P<0.01)。综上,祁连山地区小生境内3种草地土壤微生物群落存在差异,有机质碳、全磷、土壤含水量是影响群落的主要驱动因子。
The Qilian Mountains,which have large areas of grassland vegetation,form an important ecological-zone border in Western China.This research studied the composition of the grassland bacterial community in the Qilian Mountains and its relationship with soil environmental factors to provide data to support understanding of ecological dynamics in this grassland.In this research,the structure and diversity of soil bacterial communities in the 0-10 cm soil horizon of three grassland types(alpine meadow,artificial grassland and swamp meadow)in Mole Town,Qilian Mountains,were analyzed by high-throughput sequencing technology,and the correlations between the composition of soil bacterial communities and soil physical and chemical factors were studied.It was found that:1)In swamp meadow,values for soil total nitrogen,total phosphorus,organic carbon,water content and nitrate nitrogen were significantly higher than those for alpine meadow and artificial grassland(P<0.05).2)A total of 1022446 valid sequences were measured from the three regions,and the sequences were clustered with 97%consistency.The average OTU clustering values of alpine meadow,artificial grassland and swamp meadow were 4917,5233 and 5075,respectively.3)The Shannon and Simpson diversity indexes showed no significant difference among the three grassland categories.The Chao1 index ranked artificial grassland>alpine meadow>swamp meadow(P<0.05).4)Proteobacteria,Acidobacteria,Actinobacteria and Planctomycetes were shown as the main groups at the phylum level in all three grassland types.However,at the genus level,the more abundant bacterial taxa in the different grassland catagories were separated.5)Soil organic carbon,total phosphorus and soil water content were significantly correlated with the soil bacterial community composition(P<0.01).In summary,the soil microbial communities of the three grasslands in Qilian Mountains were different.The main factors affecting microbial community composition were organic carbon,total phosphorus,and soil water content.
作者
赵文
尹亚丽
李世雄
刘燕
刘晶晶
董怡玲
苏世锋
吉凌鹤
ZHAO Wen;YIN Ya-li;LI Shi-xiong;LIU Yan;LIU Jing-jing;DONG Yi-ling;SU Shi-feng;JI Ling-he(Academy of Animal and Veterinary Science,Qinghai University,Xining 810016,China;Key Laboratory of Adaptive Management of Alpine Grassland in Qinghai Province,Qinghai University,Xining 810016,China;College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China)
出处
《草业学报》
CSCD
北大核心
2021年第12期161-171,共11页
Acta Prataculturae Sinica
基金
国家自然科学基金项目(31560660)
青海省科技项目(2019-ZJ-7070)资助。
关键词
祁连山
草地类型
土壤细菌群落
土壤理化因子
Qilian Mountains
grassland type
soil bacterial communities
soil physical and chemical properties
