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基于气相色谱-离子迁移谱技术鉴定内蒙古地区3个品种羊肉挥发性代谢物 被引量:6

Identification of Volatile Metabolites of Lame Meat from Three Ovine and Caprine Breeds in Inner Mongolia by Gas Chromatography-Ion Mobility Spectrometry
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摘要 分别选择放牧条件下6只6月龄体质量相近的乌珠穆沁羊、苏尼特羊和阿尔巴斯白绒山羊背最长肌为实验材料,采用气相色谱-离子迁移谱联用技术对3个品种羊肉中的挥发性风味代谢物进行定性及定量测定,并对图谱差异进行分析,获得3个品种羊肉的挥发性代谢物差异。运用主成分分析和偏最小二乘判别分析筛选乌珠穆沁羊肉与苏尼特羊肉、阿尔巴斯白绒山羊肉的差异挥发性代谢物。结果表明:在3种羊肉中共检测到52种挥发性风味代谢物,其中筛选并定性出乌珠穆沁羊肉与苏尼特羊肉差异代谢物7种,分别为乙酸乙酯、丙酮、乙醇、2-丁酮、丁醛、壬醛和苯甲醛;乌珠穆沁羊肉与阿尔巴斯白绒山羊肉差异代谢物7种,分别为丙酮、乙酸乙酯、2-丁酮、乙醇、苯甲醛、2-戊酮和3-羟基-2-丁酮;苏尼特羊肉与阿尔巴斯白绒山羊肉差异代谢物10种,分别为丙酮、乙醇、2-丁酮、壬醛、乙酸乙酯、异戊醛、3-羟基-2-丁酮、2-戊酮、苯甲醛和辛醛。 In this study,six grazing Ujimqin sheep,six grazing Sunit sheep and six grazing Albas white cashmere goats with similar body mass were selected and slaughtered at six months of age to collect Longissimus dorsi muscles.The volatile flavor metabolites in the meat samples were determined qualitatively and quantitatively by gas chromatography-ion mobility spectrometry(GC-IMS),and differential volatile metabolites between the three breeds were identified by principal component analysis(PCA)and partial least square discriminant analysis(PLS-DA).The results showed that 52 volatile flavor metabolites were detected in all samples,among which seven differential metabolites between Ujimqin and Sunit sheep meat were identified,namely ethyl acetate,acetone,ethanol,2-butanone,butyraldehyde,nonanal and benzaldehyde;seven differential metabolites between Ujimqin sheep and Albas white cashmere goat meat,namely acetone,ethyl acetate,2-butanone,ethanol,benzaldehyde,2-pentanone and 3-hydroxy-2-butanone;and ten differential metabolites between Sunit sheep and Albas white cashmere goat meat, namely acetone, ethanol, 2-butanone, nonanal, ethyl acetate, isovaleraldehyde, 3-hydroxy-2-butanone, 2-pentanone, benzaldehyde and octanal.
作者 刘志红 赵存 谢遇春 苏馨 郭俊涛 代东亮 秦箐 张崇妍 兰茗熙 蔡婷 永泉 王志新 李金泉 LIU Zhihong;ZHAO Cun;XIE Yuchun;SU Xin;GUO Juntao;DAI Dongliang;QIN Qing;ZHANG Chongyan;LAN Mingxi;CAI Ting;YONG Quan;WANG Zhixin;LI Jinquan(Engineering Research Center for Goat Genetics and Breeding,Inner Mongolia Autonomous Region,Key Laboratory of Mutton Sheep Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Key Laboratory of Animal Genetics,Breeding and Reproduction,Inner Mongolia Autonomous Region,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Agricultural and Animal Husbandry Technology Extension Center of Inner Mongolia Autonomous Region,Hohhot 010010,China;Animal Disease Prevention and Control Center of East Ujimqin Banner,Xilingol 026000,China)
出处 《肉类研究》 2021年第11期24-30,共7页 Meat Research
基金 “十三五”国家重点研发计划重点专项(2018YFD0502000) 内蒙古自治区科技重大专项(2020ZD0004) 内蒙古自治区双一流建设青年基金项目(QN201902 QN202001) 内蒙古自治区关键技术攻关计划项目(2020GG0030)。
关键词 气相色谱-离子迁移谱 乌珠穆沁羊 苏尼特羊 阿尔巴斯白绒山羊 挥发性代谢物 gas chromatography-ion mobility spectrometry Ujimqin sheep Sunite sheep Arbas white cashmere goats volatile metabolites
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