摘要
为了研究益生菌联合益生元对肥胖的改善效果及其潜在作用机制,将植物乳杆菌(Lactobacillus plantarum)F3-2与甘露低聚糖(mannooligosaccharides,MOS)联合作用,干预高脂饮食诱导的肥胖小鼠。实验结束后,通过分析体质量、脂肪组织质量、血清脂质水平等指标,对益生菌与益生元联合作用后的效果进行评价。进一步对脂肪组织中脂代谢关键基因及小鼠粪便短链脂肪酸(short-chain fatty acids,SCFAs)水平进行检测分析,探究二者联合作用机制。结果表明,L.plantarum F3-2联合MOS作用能有效减轻小鼠体质量,此外脂肪组织质量、脂肪细胞大小及血清中甘油三酯含量相比MOS单独作用时均显著降低。两者联合作用有效提升了小鼠粪便中SCFAs特别是丁酸的水平,并增强了MOS对脂肪组织中脂代谢关键基因过氧化物酶体增殖物激活受体-γ(peroxisome proliferator-activated receptor-γ,PPARγ)及CCAAT/增强子结合蛋白-α(CCAAT/enhancer binding protein-α,C/EBPα)的抑制作用。L.plantarum F3-2与MOS有望作为合生元通过干预饮食结构改善肥胖病症。
To explore the possible effect of probiotics and prebiotics on obesity,Lactobacillus plantarum F3-2 and mannooligosaccharides(MOS)to intervene in obese mice.The critical genes of lipid metabolism in adipose tissue and the levels of short-chain fatty acids(SCFAs)in mouse feces were analyzed.The results showed that L.plantarum F3-2 combined with MOS could effectively reduce the body weight of mice,the weight of adipose tissue,the size of adipocytes and the content of TG in serum compared with MOS alone.The levels of SCFAs,especially butyric acid,in mouse feces effectively increased.The inhibitory effect of MOS on critical genes of lipid metabolism in adipose tissue,including peroxisome proliferator-activated receptor-γ(PPARγ)and CCAAT/enhancer binding protein-α(C/EBPα),were enhanced.L.plantarum F3-2 and MOS are expected to act as symbiotic to intervene in dietary structure and improve obesity.
作者
赵茂臻
梁曦
张喆
吕优优
王引
刘同杰
易华西
公丕民
张兰威
ZHAO Maozhen;LIANG Xi;ZHANG Zhe;LYU Youyou;WANG Yin;LIU Tongjie;YI Huaxi;GONG Pimin;ZHANG Lanwei(College of Food Science and Engineering,Ocean University of China,Qingdao 266000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第23期78-82,90,共6页
Food and Fermentation Industries
基金
山东泰山产业领军项目(tscy2020xxxx)
国家十三五重点研发项目(2018YFC0311201)。
关键词
益生菌
甘露低聚糖
短链脂肪酸
脂代谢
肥胖
probiotics
mannooligosaccharides
short-chain fatty acids
lipid metabolism
obesity
