摘要
背景:泡状棘球蚴病与细粒棘球蚴病在体内的生长方式不同,泡状棘球蚴病呈浸润性生长,可侵袭组织血管、胆管;细粒棘球蚴病具有纤维性外囊壁包裹病变,对组织呈压迫性生长。两种棘球蚴病对血管侵袭、血管形成产生的作用尚不明确。目的:探究两种棘球蚴绦虫原头节对内皮祖细胞中血管生成相关因子的影响。方法:提取、培养、鉴定C57BL/6小鼠骨髓来源内皮祖细胞,提取两种棘球蚴绦虫原头节;将两种棘球蚴绦虫原头节分别与内皮祖细胞共培养24,48,60 h,ELISA检测细胞共培养上清液中血管内皮生长因子A、基质细胞衍生因子1α的分泌水平;Western blot检测各组内皮祖细胞中血管生成抑制蛋白1、血管内皮生长因子A、缺氧诱导因子1α、基质细胞衍生因子1α蛋白的相对表达量。结果与结论:①泡状棘球蚴组共培养60 h时分泌血管内皮生长因子A、基质细胞衍生因子1α水平明显高于细粒棘球蚴组(P<0.05);②泡状棘球蚴组共培养48,60 h时内皮祖细胞中血管生成抑制蛋白1、缺氧诱导因子1α、基质细胞衍生因子1α蛋白的相对表达量高于细粒棘球蚴组(P<0.05);③泡状棘球蚴组与细粒棘球蚴组共培养48,60 h的血管内皮生长因子A相对表达量无显著差异(P>0.05);④结果表明,泡状棘球蚴促进血管生成,细粒棘球蚴对血管生成影响小。
BACKGROUND:Alveolar echinococcosis and cystic echinococcosis grow in different ways in the body.Alveolar echinococcosis is infiltrating growth,which can invade tissues,blood vessels,and bile ducts.Cystic echinococcosis has fibrous outer cyst wall-wrapped lesions,which show compressive growth of tissues.The effect of the two types of hydatidosis on the invasion and formation of blood vessels is still unclear.OBJECTIVE:To explore the effects of two kinds of Echinococcus granulosus protoscolex on angiogenesis-related factors in endothelial progenitor cells.METHODS:After extraction,culture,and identification of endothelial progenitor cells from C57BL/6 mouse bone marrow,and extraction of two types of Echinococcus granulosus protoscolex,two kinds of Echinococcus granulosus protoscolex were co-cultured with endothelial progenitor cells for 24,48,and 60 hours.ELISA was applied to detect the secretion of vascular endothelial growth factor A and stromal cell-derived factor-1αin the supernatant of cell co-culture.Western blot assay was utilized to detect the relative expression of vasohibin-1,vascular endothelial growth factor A,hypoxia-inducible factor 1α,and stromal cell-derived factor-1αprotein in endothelial progenitor cells of each group.RESULTS AND CONCLUSION:(1)The levels of vascular endothelial growth factor A and stromal cell-derived factor-1αwere significantly higher in the alveolar echinococcosis group than those in the cystic echinococcosis group at 60 hours of coculture(P<0.05).(2)Relative expression levels of vasohibin-1,hypoxia-inducible factor 1α,and stromal cell-derived factor-1αwere higher in the alveolar echinococcosis group than those in the cystic echinococcosis group at 48 and 60 hours of coculture(P<0.05).(3)The relative expression levels of vascular endothelial growth factor A were not significantly different in the alveolar echinococcosis and cystic echinococcosis groups at 48 and 60 hours of coculture(P>0.05).(4)It is concluded that alveolar echinococcosis promotes angiogenesis,and cystic echinococcosis has little effect on angiogenesis.
作者
俞晓凡
姜慧娇
谭小武
吴向未
Yu Xiaofan;Jiang Huijiao;Tan Xiaowu;Wu Xiangwei(School of Medicine,Shihezi University,Shihezi 832008,Xinjiang Uygur Autonomous Region,China;Department of General Surgery,First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi 832008,Xinjiang Uygur Autonomous Region,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第24期3892-3896,共5页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81760570),项目负责人:吴向未。
