摘要
目的研究白鲜碱对皮肤浅部真菌红色毛癣菌的体外抑菌作用。方法采用液基稀释法测定白鲜碱对红色毛癣菌的最低抑菌浓度(MIC);采用高通量测序技术对白鲜碱(25.0、12.5μg/mL)作用的红色毛癣菌进行转录组测序,对测序序列进行处理和生物学信息分析,进行差异表达基因、基因本体论(GO)功能显著性和京都基因与基因组百科全书(KEGG)富集分析等。结果液基稀释法结果表明,白鲜碱对红色毛癣菌的MIC为50μg/mL。高通量测序结果显示,对照组与白鲜碱25.0μg/mL组比较,差异基因总数为890个;其中上调表达384个,下调表达542个。白鲜碱作用后红色毛癣菌的差异基因主要表现于细胞膜结构、膜固有成分、氧化还原酶活性、对药物的反应和细胞色素复合体等。白鲜碱作用后红色毛癣菌在代谢途径、MAPK信号通路、ABC转运蛋白合成、色氨酸代谢等富集通路相关基因均有显著性改变(P<0.05)。结论白鲜碱对红色毛癣菌的抑菌机制可能与抑制细胞膜结构的完整性、影响红色毛癣菌的能量代谢、降低耐药性等途径有关。
Objective To study the antibacterial effect of dictamnine on Trichophyton rubrum. Methods The minimal inhibitory concentration(MIC) of dictamnine against Trichophyton rubrum was determined by liquid based dilution method. The transcriptome of Trichophyton rubrum treated with dictamnine was sequenced by high-throughput sequencing technology, and the sequencing sequence was processed and the biological information was analyzed to analyze the differentially expressed genes, the significance of GO function and KEGG metabolic pathway. Results the MIC of dictamnine against Trichophyton rubrum was 50 μg/mL. The results of high-throughput sequencing showed that there were 890 differentially expressed genes in the control group compared with the high-dose group, including 384 up-regulated genes and 542 down regulated genes. The differential genes of Trichophyton rubrum treated with dictamnine were mainly expressed in cell membrane structure, membrane intrinsic components, oxidoreductase activity, drug response and cytochrome complex. There were significant changes in metabolic pathway, MAPK signaling pathway,ABC transporter synthesis and tryptophan metabolism of Trichophyton rubrum after dictamnine treatment(P < 0.05). Conclusion The antibacterial mechanism of dictamnine against Trichophyton rubrum may be related to inhibiting the integrity of cell membrane structure, affecting the energy metabolism of Trichophyton rubrum and reducing drug resistance.
作者
刘登宇
高璐
吴建美
尉迟邈
曲琳
LIU Dengyu;GAO Lu;WU Jianmei;YU Chimiao;QU Lin(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014000,China;Inner Mongolia Medical University,Hohhot 010107,China;Inner Mongolia Center for Comprehensive Disease Control and Prevention,Hohhot 010020,China)
出处
《药物评价研究》
CAS
2021年第12期2563-2570,共8页
Drug Evaluation Research
基金
内蒙古自治区科技计划项目(201802117)。
关键词
白鲜碱
红色毛癣菌
高通量测序技术
抑菌
dictamnine
Trichophyton rubrum
high-throughput sequencing
antibacterial
