摘要
目的了解无偿献血者中戊型肝炎病毒(HEV)的实际感染状况及核酸筛查的可行性与实用性。方法选取2021年4月~2021年5月金华市中心血站1 017名无偿献血者的血液标本,采用核酸检测方法(NAT)ChiTaS BSS1200血液核酸检测系统检测其HEV RNA,对其中阳性的标本再采用酶联免疫吸附法(ELISA)检测其IgM抗-HEV和IgG抗-HEV,并采用基因测序法做HEV基因分型。结果本组无偿献血者HEV RNA阳性检出率0.98‰(1/1 017),该个体IgM和IgG抗-HEV均为阴性,其HEV基因4型。结论无偿献血人群中确实存在HEV RNA阳性感染者;NAT筛查献血者HEV RNA是可性和有效的。
Objective To investigate the prevalence of hepatitis E virus(HEV) among voluntary blood donors, and the viability and practicability of nucleic acid testing(NAT) in Jinhua area. Methods Blood samples from 1 017 voluntary blood donors at Jinhua Blood Center from April to May 2021 were collected, and HEV RNA was detected by CHURAS BSS1200 blood nucleic acid detection system. The reactive samples were re-tested for IgM and IgG antibodies by ELISA, and HEV genotyping by gene sequencing.Results The yield rate of HEV RNA out of voluntary blood donors in Jinhua was 0.98 ‰(1/017). The individual was negative for IgM and IgG antibodies, but was confirmed to be HEV RNA genotype 4 by sequencing.Conclusion HEV RNA positive donors have been found in this study. HEV RNA blood screening can effectively enhance the safety of blood transfusion.
作者
杜晓明
蔡隽
华重千
牛鸿婧
钱江
DU Xiaomin;CAI Jun;HUA Chongqian;NIU Hongjing;QIAN Jiang(Jinhua Blood Center,Jinhua 321000,China)
出处
《中国输血杂志》
CAS
2021年第11期1197-1199,共3页
Chinese Journal of Blood Transfusion
基金
金华市科技计划项目(2020-4-087)。
