摘要
为研究红景天苷对运动疲劳大鼠骨骼肌线粒体自由基代谢及呼吸链功能的影响,将健康雄性Wistar大鼠随机分为空白对照组(A组)、运动模型组(B组)、红景天苷低剂量运动组[50 mg·(kg·d)^(-1),C组]、中剂量运动组[100 mg·(kg·d)^(-1),D组]、高剂量运动组[200 mg·(kg·d)^(-1),E组]。除A组外,其余各组大鼠进行递增负荷跑台运动建立运动疲劳模型,记录大鼠体重及末次跑台力竭时间,检测骨骼肌线粒体锰超氧化物歧化酶(Mn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平及呼吸链酶复合体Ⅰ—Ⅳ(CⅠ—CⅣ)活性,采用蛋白免疫印迹法检测骨骼肌Kelch样ECH相关蛋白1(Keap1)、核呼吸因子2(Nrf2)及血氧红素加氧酶(HO-1)表达水平。结果表明:①与A组相比,B组大鼠跑台力竭运动时间,Mn-SOD、GSH-Px、CⅠ—CⅣ活性及Keap1、Nrf2、HO-1蛋白表达均显著降低,MDA含量显著升高(P<0.01)。②与B组相比,D、E组大鼠跑台力竭时间、Mn-SOD活性显著增加(P<0.01),E组大鼠MDA含量显著降低(P<0.01);C、D、E组GSH-Px、CⅢ、CⅣ活性及Keap1、Nrf2、HO-1蛋白表达量显著增加(P<0.01或0.05),D、E组CⅠ活性显著增加(P<0.01或0.05),E组CⅡ活性显著增加(P<0.05)。红景天苷可通过提升机体抗氧化防御能力及线粒体呼吸链中相关酶活性,缓解运动性疲劳的产生,其机制可能是通过激活Keap1-Nrf2-ARE通路来实现的。
To study the influence of salidroside on mitochondrial free radical metabolism and respiratory chain function in rats with exercise fatigue,healthy male Wistar rats were randomly divided into blank control group(group A),exercise model group(group B),salidroside low dose exercise group[50 mg·(kg·d)^(-1),group C],middle dose exercise group[100 mg·(kg·d)^(-1),group D]and high dose exercise group[200 mg·(kg·d)^(-1),group E,].In addition to group A,the rest of the rats were subjected to incremental treadmill exercise to establish the exercise fatigue model.The body weight and the last time of treadmill exhaustion were recorded.The levels of manganese superoxide dismutase(Mn-SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA)and the activities of respiratory chain enzyme complexes Ⅰ—Ⅳ(CⅠ—CⅣ)in skeletal muscle mitochondria were detected.The protein expression levels of Keap1,Nrf2 and HO-1 in skeletal muscle were detected.The results showed that①Compared with group A,the time of treadmill exhaustive exercise,the activities of Mn-SOD and GSH-Px,the activities of respiratory chain enzyme complexes Ⅰ—Ⅳ,the protein expressions of Keap1,Nrf2 and HO-1 in group B were significantly decreased,and the content of MDA was significantly increased(P<0.01).②Compared with group B,the treadmill exhausting time,the activitie of Mn-SOD in group D and group E were significantly increased,the content of MDA in group E was significantly decreased(P<0.01),the activities of CⅢ and CⅣ,the activitie of GSH-Px,and the protein expression of Keap1,Nrf2 and HO-1 in group C,D and E were significantly increased(P<0.01 or 0.05),the activities of CⅠ in group D and group E were significantly increased(P<0.01 or 0.05),and the activities of CⅡ in group E were significantly increased(P<0.05).Salidroside can alleviate exercise-induced fatigue by enhancing antioxidant defense capacity and related enzyme activities in mitochondrial respiratory chain,which may be realized by activating Keap1-Nrf2-HO-1 pathway.
作者
杨芳芳
YANG Fangfang(Henan University of Economics and Law,Zhengzhou 450046,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2021年第5期61-66,共6页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
河南省社科联调研课题“新时代高校体育美育改革发展研究”(SKL-2020-2263)。
关键词
红景天苷
运动
氧化应激
呼吸链酶复合体
蛋白质表达
salidroside
exercise
oxidative stress
respiratory chain enzyme complex
protein expression
