摘要
为探讨云南核桃丛枝病感病植株内源激素含量的变化情况,采用LC-MS/MS法测定云南核桃主产区楚雄、大理、临沧三地的感病和健康植株叶片中的24种内源激素的含量。共检测到11种内源激素,含量变化最大的是细胞分裂素类激素,该类激素在感病植株中的含量相对于健康植株均成倍增加,其中增加最多的是cZ,3个核桃主产区的感病植株cZ含量均为健康植株的3倍左右,且C/A值在感病叶中均高于健康叶;其次是ABA,感病植株ABA含量为健康植株的2倍左右;赤霉素类的GA_(7)仅仅在感病植株中被检测到。结果表明,核桃感染丛枝病后植株内源激素含量发生明显变化,表现为细胞分裂素增加,从而导致丛枝、叶片卷曲变小、腋芽萌生等丛枝病症状。
In order to investigate the changes of endogenous hormones in susceptible Juglans siggillata infected by phytoplasma,twenty four endogenous hormone contents in the leaves of susceptible and healthy plants from three main Juglans siggillata producting areas(Chuxiong,Dali and Lincang) in Yunnan were determined by LC-MS/MS.Eleven kinds of endogenous hormones were detected,among which the content of cytokinin were the most variable.Compared with healthy Juglans siggillata,the content of cytokinin in susceptible Juglans siggillata increased exponentially.Among them,the most increased was cZ.The cZ content of susceptible plants in the three main producting areas is about three times that of healthy plants,and the C/A value of susceptible leaves is higher than that of healthy leaves.The content of abscisic acid(ABA) in susceptible plants was about twice that of healthy plants;GA_(7) of gibberellins was only detected in susceptible plants.The results showed that the endogenous hormone content of Juglans siggillata infected with witches’ broom disease changed significantly,which showed that the increase of cytokinin and led to witches’ broom disease such as leaf curling and diminishing,and axillary bud sprouting.
作者
韩颖
马建鹏
张静
梁晨
杨斌
赵宁
HAN Ying;MA Jianpeng;ZHANG Jing;LIANG Chen;YANG Bin;ZHAO Ning(School of Life Science,Southwest Forestry University,Kunming 650224,China;Dali Bai Autonomous Prefecture Forestry and Grassland Pest Control and Quarantine Bureau,Dali Yunnan 671000,China;Laboratory of Forest Disaster Warning and Control in Yunnan Province,Southwest Forestry University,Kunming 650224,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2021年第12期1898-1904,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
云南省林业科技创新项目[(2016)CX01]
云南省林业科技推广项目(2018ts03)。
关键词
云南核桃
丛枝病
内源激素
液相色谱串联质谱法
Juglans siggillata
Witches’broom disease
Endogenous hormones
Liquid chromatography tandem mass spectrometry
