摘要
目的:研究长春胺对非动脉炎性前部缺血性视神经病变(NAION)大鼠模型的潜在神经保护作用。方法:90只SD大鼠随机分为正常组、单纯模型组、造模羧甲基纤维素钠灌胃组(NaCMC)组、长春胺组、造模PI3K抑制剂LY294002玻璃体腔注射(IVT LY)+长春胺组、造模DMSO玻璃体腔注射(IVT DMSO)+长春胺组。对各组进行眼底照相,眼底荧光血管造影,血清一氧化氮(NO)浓度检测,HE染色观察视网膜形态,甲苯胺蓝染色观察视神经轴突形态,免疫荧光染色计数视网膜神经节细胞(RGCs),实时定量PCR检测p-AKT、eNOS含量。结果:rNAION大鼠(rNAION)造模成功后第1天即可见大鼠视盘水肿,边界模糊。造模后第28天,长春胺组的NO浓度显著高于正常对照组、单纯模型组及NaCMC组。与单纯模型组相比,长春胺组RGCs数量明显增加(P<0.05),p-AKT、eNOS含量明显增加(P<0.05)。IVT LY+长春胺组RGCs数量及p-AKT、eNOS含量较长春胺组明显减少(P<0.05)。结论:长春胺可能通过PI3K/AKT/eNOS信号通路发挥视神经保护作用,有望成为NAION的有效治疗药物。
Objective:To study the potential neuroprotective effect of Vincamine in a rat model of non-arteritic anterior ischemic optic neuropathy(NAION).Methods:Ninety SD rats were randomly divided into six groups,including normal control group,simple model group,carboxymethylcellulose sodium(NaCMC)group,Vincamine group,intravitreal injection of PI3K inhibitor LY294002(IVT LY)+Vincamine group,and intravitreal injection of DMSO(IVT DMSO)+Vincamine group.Fundus photography,fundus fluorescein angiography,measurement of serum nitric oxide(NO)concentration,hematoxylin and eosin(HE)staining to observe the morphology of the retina,toluidine blue staining to observe the morphology of optic nerve axons,flat-mount immunofluorescence to count retinal ganglion cells(RGCs),and real-time quantitative PCR to detect the expression of p-AKT and eNOS were performed in all the six groups.Results:Optic disc edema and blurred optic disc borders were seen in the rat model of NAION(rNAION)on the first day after photodynamic induction.28 days after induction,the Vincamine group had a higher NO concentration than the normal control group,simple model group,and NaCMC group,respectively.Compared with that respectively in simple model group,the number of RGCs in Vincamine group increased significantly(P<0.05),and the expression of p-AKT and eNOS increased significantly(P<0.05).The number of RGCs and the expression of p-AKT and eNOS in IVT LY+Vincamine group were significantly reduced as com-pared with those in Vincamine group(P<0.05).Conclusion:Vincamine may exert optic neuroprotection through the PI3K/AKT/eNOS signaling pathway and may become an effective drug for NAION.
作者
付梅
李璐
梁超群
苏钰
刘珏君
陈长征
FU Mei;LI Lu;LIANG Chaoqun;SU Yu;LIU Juejun;CHEN Changzheng(Eye Center,Renmin Hospital of Wuhan University,Wuhan 430060,Hubei,China)
出处
《武汉大学学报（医学版）》
CAS
2022年第1期34-39,共6页
Medical Journal of Wuhan University
基金
国家自然科学基金青年基金资助项目(编号:81600740)
武汉大学自主科研基金资助项目(编号:2042019kf0071)。
