摘要
以木瓜皮为原料,研究了木瓜皮多酚和黄酮的提取工艺及抗氧化、酪氨酸酶和胰脂肪酶抑制活性。在单因素实验基础上采用正交试验研究超声温度、超声时间、乙醇浓度、料液比对木瓜皮多酚和黄酮含量的影响,并测定木瓜皮对DPPH自由基和ABTS自由基的清除能力以及对酪氨酸酶和胰脂肪酶的抑制活性。结果表明,木瓜皮多酚和黄酮的最佳提取条件为:超声温度40℃,超声时间60 min,乙醇浓度60%,料液比1:25 g/mL,在此条件下多酚和黄酮含量分别为(82.00±0.65)mg/g和(162.76±2.82)mg/g。抗氧化活性实验结果表明,木瓜皮提取物对DPPH自由基和ABTS自由基的清除率分别为(94.79%±0.10%)和(96.94%±0.23%)。抑制酶活性实验结果表明,木瓜皮提取物对分别以L-Tyr和L-Dopa为底物的酪氨酸酶的抑制率为(83.33%±6.80%)和(67.12%±0.32%),对胰脂肪酶的抑制率为(82.78%±1.28%),说明木瓜皮具有较强的抗氧化能力、酪氨酸酶及胰脂肪酶抑制活性。
The extract process of polyphenols and flavonoids from papaya peel were optimized and their antioxidant activity, anti-tyrosinase and anti-pancreatic lipase were evaluated. On the basis of single factor experiment, orthogonal experiment was used to study the influence of ultrasonic temperature, ultrasonic time, ethanol concentration and solid-liquid ratio on the contents of polyphenols, flavonoids from papaya peel. The scavenging ability of papaya peel on DPPH free radicals and ABTS free radicals and its inhibitory activity on tyrosinase and pancreatic lipase were measured. The optimal extraction conditions were obtained: Ultrasonic temperature 40 ℃, ultrasonic time 60 min, 60% ethanol, solid-liquid ratio1:25 g/mL. Under the optimal conditions, the contents of polyphenols and flavonoids from papaya peel reached(82.00±0.65) mg/g and(162.76±2.82) mg/g, respectively. The scavenging rates of papaya peel on DPPH free radicals and ABTS free radicals reached(94.79%±0.10%) and(96.94%±0.23%), respectively. The inhibition rate of papaya peel on tyrosinase with L-Tyr and L-Dopa as the substrate reached(83.33%±6.80%) and(67.12%±0.32%), respectively. The inhibition rate of papaya peel on pancreatic lipase reached(82.78%±1.28%), indicating that papaya peel had strong antioxidant capacity, tyrosinase and pancreatic lipase inhibitory activity.
作者
裴文清
吕泸楠
王靖宇
浦思琦
雷霜
王春丽
PEI Wenqing;LV Lunan;WANG Jingyu;PU Siqi;LEI Shuang;WANG Chunli(School of Pharmacy,East China University of Science and Technology,Engineering Research Center of Pharmaceutical Process Chemistry,Ministry of Education,Shanghai Key Laboratory of New Drug Design,Shanghai 200237,China)
出处
《食品工业科技》
CAS
北大核心
2022年第1期188-195,共8页
Science and Technology of Food Industry
基金
国家科技重大专项(2018ZX09735002)。
关键词
木瓜皮
多酚
黄酮
抑制酪氨酸酶
抑制胰脂肪酶
papaya peel
polyphenols
flavonoids
anti-tyrosinase
anti-pancreatic lipase
