摘要
目的基于磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(Akt)通路探究丙泊酚对七氟烷诱导大鼠神经细胞凋亡的保护作用。方法2020年1月—2021年1月于湖北省恩施土家族苗族自治州中心医院实验室进行实验,选取健康、清洁级Wistar雄性大鼠30只,随机数字表法分为对照组、七氟烷组、七氟烷+丙泊酚组(联合组),各10只。七氟烷组吸入1.5%七氟烷干预,联合组在七氟烷组基础上加丙泊酚150 mg/kg腹腔注射,对照组给予等体积的生理盐水干预,均连续干预2周。干预后各组大鼠行Morris水迷宫实验,观察大鼠海马神经细胞凋亡情况,检测氧化应激相关指标、凋亡相关因子及PI3K/Akt通路蛋白表达水平。结果逃避潜伏期、海马神经细胞凋亡率比较,七氟烷组>联合组>对照组(F/P=17.038/0.001、36.834/0.001),穿越平台次数、目标象限停留时间占比比较,七氟烷组<联合组<对照组(F/P=8.913/0.001、9.125/0.001)。LDH、MDA、Bax、Caspase-3水平比较,七氟烷组>联合组>对照组(F/P=35.166/0.001、20.773/0.001、43.896/0.001、17.462/0.001),SOD、GSH-Px、Bcl-2水平及PI3K、p-Akt表达比较,七氟烷组<联合组<对照组(F/P=12.092/0.001、16.243/0.001、15.236/0.001、28.850/0.001、28.869/0.001)。结论丙泊酚能改善七氟烷诱导的大鼠认知功能、氧化应激反应,抑制神经细胞凋亡,可能与PI3K/Akt通路有关。
Objective To explore the protective effect of propofol on sevoflurane induced neuronal apoptosis in rats based on phosphatidylinositol 3 hydroxykinase(P13K)/protein kinase B(Akt)pathway.Methods From January 2020 to January 2021,experiments were carried out in the laboratory of the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Hubei Province.30 healthy and clean Wistar male rats were selected and divided into control group,sevoflurane group,and sevoflurane group by random number table.Sevoflurane+propofol group(combined group),each with 10 rats.The sevoflurane group was treated by inhalation of 1.5%sevoflurane,and the combination group was given an intraperitoneal injection of 150 mg/kg propofol on the basis of the sevoflurane group.The control group was given an equal volume of normal saline for intervention,both for continuous intervention for 2 weeks.After intervention,each group of rats underwent Morris water maze experiment to observe the apoptosis of rat hippocampal nerve cells,and detect oxidative stress-related indicators,apoptosis-related factors and PI3K/Akt pathway protein expression levels.Results Comparison of escape latency and hippocampal neuronal apoptosis rate,sevoflurane group>combined group>control group(F/P=17.038/0.001,36.834/0.001),the number of times of crossing the platform and the percentage of stay time in the target quadrant,sevoflurane Group<Combination Group<Control Group(F/P=8.913/0.001,9.125/0.001).Comparison of LDH,MDA,Bax,Caspase3 levels,sevoflurane group>combination group>control group(F/P=35.166/0.001,20.773/0.001,43.896/0.001,17.462/0.001),SOD,GSH Px,Bcl2 levels and Comparison of PI3K and p Akt expressions,sevoflurane group<combination group<control group(F/P=12.092/0.001,16.243/0.001,15.236/0.001,28.850/0.001,28.869/0.001).Conclusion Propofol can improve the cognitive function and oxidative stress response of rats induced by sevoflurane,and inhibit neuronal apoptosis,which may be related to the PI3K/Akt pathway.
作者
张伟
朱贤林
吴帮林
朱荣誉
潘琴
梅荣
Zhang Wei;Zhu Xianglin;Wu Banglin;Zhu Rongyu;Pan Qin;Mei Rong(Department of Anesthesio-logy,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Hubei Province,Enshi 445000,China)
出处
《疑难病杂志》
CAS
2022年第1期80-83,89,共5页
Chinese Journal of Difficult and Complicated Cases
基金
湖北省自然科学基金计划项目(ZRMS2016001263)。
