摘要
目的探究二陈汤治疗肥胖型多囊卵巢综合征和肥胖型不孕的潜在机制,为“异病同治”提供理论依据。方法利用TCMSP数据库获取二陈汤的活性成分与靶点,利用Genecard数据库获取肥胖型多囊卵巢综合征和肥胖型不孕的靶点。利用Cytoscape 3.7.1构建活性成分靶点网络,利用STRING获取蛋白互作网络与核心靶点。利用Cytoscape 3.7.1与线上软件进行GO与KEGG富集分析。结果获得二陈汤成分125个,靶基因218个;肥胖型不孕靶基因2783个,肥胖型多囊卵巢综合征靶基因2962个。二陈汤治疗肥胖型不孕和肥胖型多囊卵巢综合征的共有靶基因117个,体现了“异病同治”的原则;潜在的活性成分包括槲皮素、山奈酚、柚皮素、黄芩苷和刺芒柄花素。蛋白互作网络示STAT3、JUN、AKT1、MAPK3、MAPK1、MAPK14、IL-6和FOS为“异病同治”的核心靶点。分子对接结果显示,槲皮素、山奈酚等成分与JUN具有较好的结合能力。GO与KEGG富集分析显示,AGE-RAGE信号通路、IL-17信号通路、内分泌抵抗可能是二陈汤“异病同治”的关键通路。结论二陈汤“异病同治”肥胖型多囊卵巢综合征和肥胖型不孕涉及相同靶点以及通路,可为以后的实验研究提供参考。
Objective To explore the potential mechanism of Erchen decoction in the treatment of obese polycystic ovary syndrome and obese infertility,in order to provide theoretical basis for“treating different diseases with same method”.Methods The active ingredients and targets of Erchen decoction were obtained from TCMSP database,and the targets of obese polycystic ovary syndromes and obese infertility were obtained from GeneCard database.Active ingredient-target network was constructed by Cytoscape 3.7.1,and protein-protein interaction network and core target were obtained from STRING.GO and KEGG enrichment analysis were performed by Cytoscape 3.7.1 and online software.Results 125 ingredients and 218 targets of Erchen decoction were obtained.There were 2783 target genes for obese infertility and 2962 target genes for obese polycystic ovary syndrome.Erchen decoction has a total of 117 target genes in the treatment of obese infertility and obese polycystic ovary syndromes,which proves the principle of“treating different diseases with same method”.Potential active ingredients include quercetin,kaempferol,naringin,baicalin and formononetin.PPI showed that STAT3,JUN,AKT1,MAPK3,MAPK1,MAPK14,IL-6 and FOS were the core targets of“treating different diseases with same method”.Molecular docking results showed that quercetin,kaempferol,etc.had good binding ability with JUN.GO and KEGG enrichment analysis showed that AGE-RAGE signaling pathway,IL-17 signaling pathway and endocrine resistance might be the key pathways for the“treating different diseases with same method”of Erchen decoction.Conclusion Erchen decoction treating"different diseases with same method"involves same targets and same pathways,which can provide reference for future experimental research.
作者
蔡孟成
金永生
俞超芹
程雯
俞瑾
周玲
CAI Mengcheng;JIN Yongsheng;YU Chaoqin;CHENG wen;YU Jin;ZHOU Ling(School of Basic Medicine,Naval Medical University,Shanghai 200433,China;School of Pharmacy,Naval Medical University,Shanghai 200433,China;College of Traditional Chinese Medicine,Naval Medical University,Shanghai 200433,China)
出处
《药学实践杂志》
CAS
2022年第1期20-27,52,共9页
Journal of Pharmaceutical Practice
基金
国家自然科学基金(81973896,82004408)
上海市科学技术委员会科研计划项目(19401930200)
上海市青年科技英才杨帆计划项目(20YF1448600)。
关键词
痰湿
不孕症
多囊卵巢综合征
分子对接
网络药理学
phlegm-dampness
infertility
polycystic ovary syndrome
molecular docking
network pharmacology
