摘要
目的比较两种人类白细胞抗原B27(HLA-B27)检测试剂盒临床检测效果与异常结果分析,为临床提供可靠的检测报告。方法采用HLA-B27-FITC/HLA-B7-PE和HLA-B27-FITC/CD3-PE两种检测试剂盒检测1208例腰椎病、颈椎病、关节痛、脊柱僵硬和强直性脊柱炎患者标本中HLA-B27的表达,结合临床诊断分析比较两种方法的检测结果。结果HLA-B27-FITC/HLA-B7-PE检测试剂盒在检测1208份标本中有12份(0.99%)HLA-B7+HLA-B27+细胞群的百分比≥90%,判读为HLA-B27疑似阳性,而HLA-B27-FITC/CD3-PE试剂盒检测结果显示均为阳性结果;另外,淋巴细胞群分群不清晰易导致HLA-B27-FITC/HLA-B7-PE试剂盒将HLA-B27阳性结果判读为假阴性,而HLA-B27-FITC/CD3-PE试剂盒利用CD3设门可以避免假阴性的出现。结论两种检测试剂盒检测HLA-B27结果均准确可靠,且各具特点。
Objective To compare the clinical detection effects and abnormal results analysis of two human leucocyte antigen(HLA)-B27 detection kits in order to provide a reliable laboratory report for clinical diagnosis.Methods A total of 1208 samples were tested by two reagents,including HLA-B27-FITC/HLA-B7-PE kit and HLA-B27-FITC/CD3-PE kit,and combined with clinical diagnosis,the results of the two methods were compared.Results Totally 12(0.99%)of 1208 patients detected by HLA-B27-FITC/HLA-B7-PE detection kit showed the percentage of HLA-B7+HLA-B27+cell population≥90%,and they were interpreted as HLA-B27 suspected positive,while HLA-B27-FITC/CD3-PE kit test results were all positive.In addition,unclear lymphocyte grouping was easy to cause HLA-B27-FITC/HLA-B7-PE kit to interpret HLA-B27 positive results as false negative,while the HLA-B27-FITC/CD3-PE kit could avoid false negative by using CD3 gate.Conclusion The results of HLA-B27 detection by the two detection kits are accurate and reliable,and each has its own characteristics.
作者
陈永强
郑璐
张玥
王杜平
程星
陈艳红
周忠海
牛国平
CHEN Yongqiang;ZHENG Lu;ZHANG Yue;WANG Duping;CHENG Xing;CHEN Yanhong;ZHOU Zhonghai;NIU Guoping(Department of Clinical Laboratory,Xuzhou Central Hospital,Xuzhou,Jiangsu 221009,China;Department of Experiment,Huaihai Hospital Affiliated to Xuzhou Medical University,Xuzhou,Jiangsu 221004,China)
出处
《国际检验医学杂志》
CAS
2022年第2期152-155,共4页
International Journal of Laboratory Medicine
基金
江苏省双创博士项目(2020SC01)
徐州市重点研发计划社会发展项目(KC20110)
徐州市中心医院课题(2-2109024)。
