摘要
为增加薰衣草残渣的利用价值,优化薰衣草中总三萜提取工艺并测定其体外抗氧化活性。以薰衣草中总三萜的提取量为评价指标,采用单因素实验和响应面法优化薰衣草总三萜超声辅助提取工艺,得到最优工艺为料液比为1:33(g/mL),乙醇浓度为89%,超声时间为60 min,超声功率为70%(445 W),理论提取量为39.64 mg/g,在该条件,平行验证三次,实际提取量为(39.95±0.32)mg/g与预测值相近。并对薰衣草总三萜进行体外抗氧化活性测定,以V_(C)作为对照,分别对薰衣草总三萜的DPPH自由基清除率、OH自由基清除率和O_(2)^(-)自由基清除率进行测定,其清除率分别为86.83%、42.06%、47.93%。通过超声辅助提取工艺条件优化,薰衣草中总三萜提取量明显提高,并具有较强的抗氧化能力,为薰衣草残渣的再利用提供理论前提,以进一步挖掘利用空间。
In order to increase the utilization value of Lavandula angustifolia(LA)residue and provide a theoretical basis for the development and utilization of LA waste,the extraction process of total triterpenoids from LA was optimized and its antioxidant activity was determined in vitro.Taking the yield of total triterpenes in LA as the evaluation index,the single factor experiments and the response surface methodology were used to optimize the extraction process of ultrasonic-assisted extraction of total triterpenes from LA.The results showed that the optimum extraction conditions were ethanol concentration 89%,ratio of material to liquid 1:33(g/mL),extraction time 60 min,and ultrasonic power 70%(445 W).The theoretical extraction volume was 39.64 mg/g,and the actual one was(39.95±0.32)mg/g under this optimum condition,which was close to the predicted value.Afterwards,using V_(C) as a control,DPPH free radical,OH free radical,and O_(2)^(-) free radical scavenging experiments were subjected to measure the anti-oxidant activity of total triterpenes,and their scavenging rates were 86.83%,42.06% and 47.93%,respectively.After the optimization of the ultrasonic-assisted extraction process conditions,the total triterpenes extracted from LA is significantly increased,and the extract has strong antioxidant activity,which provides a theoretical basis for the reuse of LA waste to further tap the potential value.
作者
尼格尔热依·亚迪卡尔
邓淑萍
白玉
古丽皮耶·吾普尔
NIGARY Yadikar;DENG Shuping;BAI Yu;GULIPIE Upur(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《食品工业科技》
CAS
北大核心
2022年第3期172-179,共8页
Science and Technology of Food Industry
关键词
薰衣草
总三萜
响应面
抗氧化
Lavandula angustifolia
total triterpenes
response surface
antioxidant
