摘要
原生质体融合技术在食用菌遗传育种中具有重要应用价值,它能够克服种间、属间甚至科间的屏障,实现远缘融合,构建出新型菌株,对食用菌菌种改良、新品种选育、珍稀野生食用菌驯化具有十分重要的意义。文章综述了食用菌原生质体制备和再生的影响因素、融合方法、融合子鉴定方法及育种等方面的研究进展,旨在为食用菌的遗传育种提供思路和方法依据。
In the genetic breeding of edible mushroom,protoplast fusion technology offers a lot of potential.It breaks through barriers across species,genera,and even families,allowing for the fusion and creation of new strains.It′s crucial for improving current edible mushrooms strains,developing new strains,and domesticating rare wild edible mushrooms.This paper reviewed current research progress in the influencing factors of protoplast preparation and regeneration,fusion methods,fusant identification methods and breeding of edible mushroom,in order to provide ideas and method basis for the genetic breeding of edible mushroom.
作者
李亚娇
孙国琴
郭九峰
王海燕
于传宗
庞杰
LI Yajiao;SUN Guoqin;GUO Jiufeng;WANG Haiyan;YU Chuanzong;PANG Jie(Institute of Vegetables and Flowers,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;College of Physical Science and Technology,Inner Mongolia University,Hohhot 010021,China)
出处
《北方农业学报》
2021年第6期121-127,共7页
Journal of Northern Agriculture
基金
内蒙古农牧业青年创新基金项目(2020QNJJNO3)
内蒙古农牧业创新基金项目(2019CXJJN09-1)
国家食用菌产业技术体系呼和浩特综合试验站(CARS20)
内蒙古自治区科技计划项目(2020GG0050)
内蒙古食用菌资源开发利用新技术创新人才团队。
关键词
食用菌
原生质体
制备
再生
融合方法
融合子鉴定
Edible mushroom
Protoplast
Preparation
Regeneration
Fusion method
Fusant identification