摘要
目的筛选喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)预后相关免疫核心基因并构建预后风险评分模型。方法利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中LSCC转录组测序信息,筛选差异表达基因,并与已知免疫相关基因取交集,筛选LSCC中预后相关免疫核心基因。利用单因素Cox回归构建风险评分模型并采用Kaplan-Meier法验证风险评分模型与LSCC患者预后的关系,同时绘制受试者工作特征(receiver operating characteristic,ROC)曲线验证该模型准确度。结果 LSCC组织和癌旁组织中共有6 800个差异表达基因,通过单因素Cox分析并与已知免疫相关基因取交集,从差异表达基因中筛选出34个预后枢纽基因。基于免疫核心基因构建风险评分模型,高危险组患者总生存期短于低危险组患者(P<0.01),风险评分模型预测LSCC患者预后的ROC曲线下面积为0.930。结合临床病理学参数分析发现,患者性别是LSCC预后的保护因素,N分期晚期和高风险评分为LSCC预后的危险因素(均P<0.05),而患者年龄和T分期与LSCC预后无关(均P>0.05)。结论 LSCC中存在多个免疫核心基因异常表达,且与患者预后相关,基于其构建的风险评分模型可较好预测患者预后。
Objective To screen the immune hub genes related to the prognosis of laryngeal squamous cell carcinoma(LSCC)and to construct a prognostic risk score model.Methods LSCC transcriptome sequencing information in The Cancer Genome Atlas(TCGA)database was used to screen differentially expressed genes,and the intersection of known immune-related genes was used to screen the prognostic immune hub genes in LSCC.Univariate Cox regression was used to construct the risk score model,and Kaplan-Meier method was used to verify the relationship between the risk score model and the prognosis of patients with LSCC.Meanwhile,receiver operating characteristic(ROC)curve was drawn to verify the accuracy of the model.Results A total of 6800 differentially expressed genes were screened out in LSCC tissues and adjacent tissues,and 34 prognostic hub genes were screened out from differentially expressed genes by univariate Cox analysis and intersection with known immune-related genes.The risk score model was constructed based on the immune hub genes.The overall survival time of patients in the high-risk group was significantly shorter than that in the low-risk group(P<0.01).The area under the ROC curve for predicting the prognosis of patients with LSCC by the risk score model was 0.930.In combination with clinicopathological parameters,gender was a protective factor for the prognosis of LSCC,and advanced N stage and high risk score were risk factors for the prognosis of LSCC(all P<0.05),while age and T stage were not associated with the prognosis of LSCC(both P>0.05).Conclusions Several immune-related hub genes were abnormally expressed in LSCC,and were related to the prognosis of LSCC patients.The risk score model based on immune-related hub genes can predict the prognosis of LSCC patients.
作者
邹攀
许红淼
熊雨
苏芮
罗欢
罗晶婧
蔺婷
王贤文
何迎春
Zou Pan;Xu Hongmiao;Xiong Yu;Su Rui;Luo Huan;Luo Jingjing;Lin Ting;Wang Xianwen;He Yingchun(Graduate School,Hunan University of Chinese Medicine,Changsha 410208,China;School of Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine,Changsha 410208,China;Hunan Provincial Ophthalmology and Otolaryngology Diseases Prevention and Treatment-with Traditional Chinese Medicine and Visual Function Protection Engineering and Technological Research Center,Changsha 410208,China;Department of Otolaryngology-Head and Neck Surgery,the First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
出处
《实用肿瘤杂志》
CAS
2022年第1期44-49,共6页
Journal of Practical Oncology
基金
国家自然科学基金(81874408,81973914)
湖南省自然科学基金(2019JJ40216,2020JJ5419)
中医药防治眼耳鼻咽喉疾病湖南省重点实验室开放基金(2018YZD04)
湖南中医药大学基础医学一流学科开放基金(2018JCYX08)。
关键词
喉鳞状细胞癌
免疫核心基因
风险评分模型
预后
laryngeal squamous cell carcinoma
immune hub genes
risk score model
prognosis
