摘要
目的探讨儿童肺炎支原体肺炎外周血T淋巴细胞亚群、细胞因子以及免疫球蛋白的动态变化。方法选取2018年12月至2020年5月本院收治的儿童支原体肺炎患儿50例作为观察组,按病情的发展进程分为急性期组和恢复期组,各25例;另选取同期健康儿童50名作为对照组。3组均进行样本采集,比较3组外周血T淋巴细胞亚群、细胞因子以及免疫球蛋白的动态效果。结果检测后,急性期组CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均低于恢复期组和对照组(P<0.05),NK细胞的比例高于恢复期组和对照组(P<0.05),恢复期组CD8^(+)与B细胞的占比与对照组比较差异无统计学意义。检测后,急性期组IFN-γ、IL-4、Th1/Th2含量均高于恢复期组和对照组(P<0.05),恢复期组IFN-γ、IL-4、Th1/Th2与对照组比较差异无统计学意义。检测后,恢复期组血清IgG水平高于急性期组和对照组(P<0.05),急性期组血清IgG水平与对照组比较差异无统计学意义,急性期组和恢复期组血清IgA水平均低于对照组(P<0.05),急性期组血清IgA水平与恢复期组比较差异无统计学意义,急性期组血清IgM水平高于对照组和恢复期组(P<0.05),对照组血清IgM水平与恢复期组比较差异无统计学意义。结论患儿感染支原体肺炎时,机体内免疫功能发生紊乱,随病情的发展,免疫状态发生变化,临床上对幼儿采取免疫监测,可对疾病的诊断以及后期治疗提供依据。
Objective To explore the dynamic analysis of peripheral blood T lymphocyte subsets,cytokines and immunoglobulins in children with Mycoplasma pneumoniae pneumonia.Methods 50 children with Mycoplasma pneumonia admitted to our hospital from December 2018 to May 2020 were selected as the observation group.According to the progression of the disease,they were divided into acute phase group and recovery phase group,with 25 cases in each group;another 50 healthy children in the same period were selected as the control group.Samples were collected for the three groups and the dynamic effects of peripheral blood T lymphocyte subsets,cytokines,and immunoglobulins were compared among the three groups.Results After testing,CD3^(+),CD4^(+),CD4^(+)/CD8^(+)in the acute phase group were lower than those in the recovery phase group and control group(P<0.05),the proportion of NK cells was higher than that in the recovery phase group and control group(P<0.05),there was no significant difference in the proportion of CD8^(+)and B cells between the recovery phase group and the control group.After detection,the levels of IFN-γ,IL-4,Th1/Th2 in the acute phase group were higher than those in the recovery phase group and the control group(P<0.05),and there was no significant diference in the IFN-γ,IL-4,Th1/Th2 levels between the recovery phase group and the control group.After testing,the serum IgG level of recovery phase group was higher than that of the acute phase group and the control group(P<0.05),there was no significant difference in serum IgG level between the acute phase group and the control group,the levels of serum IgA in the acute phase group and recovery phase group were lower than those in the control group(P<0.05),there was no significant difference in serum IgA level between acute phase group and recovery phase group,the level of serum IgM in acute phase group was higher than that in control group and recovery phase group(P<0.05),there was no significant difference in serum IgM level between the control group and the recovery phase group.Conclusion When child is infected with Mycoplasma pneumonia,the body's immune function is disordered,and the immune status changes with the development of the disease.The clinical immune monitoring of the child can provide evidence for the diagnosis and later treatment of the disease.
作者
沈阳
边兴艳
李季
张鹤
SHEN Yang;BIAN Xingyan;LI Ji;ZHANG He(Department of Laboratory Medicine,Dalian Children's Hospital,Dalian,Liaoning,116000,China)
出处
《当代医学》
2022年第5期77-80,共4页
Contemporary Medicine
