摘要
目的:调查龙岩地区人类白细胞抗原B27(HLA-B27)基因亚型的分布情况,分析其与疾病临床特征的关系。方法:收集龙岩市第二医院风湿免疫科2020年6月-2021年9月就诊的强直性脊柱炎(AS)患者98例为AS组,同期健康体检者200例为健康对照组。采用聚合酶链式反应-序列特异性引物法(PCR-SSP)检测两组的HLA-B27基因及亚型。结果:AS组HLA-B27阳性率88.78%,明显高于健康对照组的8.00%(P<0.05)。AS组患者中共检测出HLA-B2702、HLA-B2704、HLA-B2705、HLA-B2708四种基因亚型,未检测到HLA-B2710。健康对照组中检测出HLA-B2702、HLA-B2704、HLA-B2705、HLA-B2708、HLA-B2710五种基因亚型。AS组和健康对照组均以HLA-B2704和HLA-B2705亚型为主;AS组较健康对照组HLA-B2704阳性率高(P<0.05)。健康对照组较AS组HLA-B2708、HLA-B2710阳性率高(P<0.05)。相较于HLA-B2704亚型,HLA-B2705亚型外周关节炎和髋关节受累比例更高,而SpA家族史、炎性腰背痛比例更低,差异均有统计学意义(P<0.05)。髋关节炎组和非髋关节炎组的起病年龄、病程、IL-6水平、首发髋关节炎占比及HLA-B2705占比比较,差异均有统计学意义(P<0.05)。结论:本地区AS患者的HLA-B27基因亚型以HLA-B2704、HLA-B2705为主,此两型与疾病临床特征关系密切,有助于AS患者的早期诊断和干预。
Objective:To investigate the distribution of human leukocyte antigen B27(HLA-B27)gene subtype in Longyan area and analyze its relationship with clinical characteristics of the disease.Method:A total of 98 patients with ankylosing spondylitis(AS)who visited rheumatology Department of Longyan Second Hospital from June 2020 to September 2021 were enrolled as the AS group,and 200 healthy subjects were enrolled as the healthy control group during the same period.HLA-B27 gene and subtype were detected by polymerase chain reaction with sequence specific primer(PCR-SSP).Result:The positive rate of HLA-B27 in AS group was 88.78%,which was significantly higher than 8.00%in healthy control group(P<0.05).Four subtypes of HLA-B*2702,HLA-B*2704,HLA-B*2705 and HLA-B*2708 were detected in AS group,but HLA-B*2710 was not detected.Five subtypes of HLA-B*2702,HLA-B*2704,HLA-B*2705,HLA-B*2708 and HLA-B*2710 were detected in the healthy control group.HLA-B*2704 and HLA-B*2705 subtypes were dominant in AS group and healthy control group;the positive rate of HLA-B*2704 in AS group was higher than that in healthy control group(P<0.05).The positive rates of HLA-B*2708 and HLA-B*2710 in healthy control group were higher than those in AS group(P<0.05).Compared with HLA-B*2704 subtype,HLA-B*2705 subtype has a higher proportion of peripheral arthritis and hip involvement,while the proportion of SpA family history and inflammatory low back pain is lower,the differences were statistically significant(P<0.05).Comparison of onset age,course of disease,IL-6 level,proportion of first-episode hip arthritis and proportion of HLA-B*2705 between hip arthritis group and non hip arthritis group,the differences were statistically significant(P<0.05).Conclusion:HLA-B27 gene subtypes of as patients in this region are mainly HLA-B*2704 and HLA-B*2705.These two subtypes are closely related to the clinical characteristics of the disease,which is helpful for the early diagnosis and intervention of as patients.
作者
李曼
谢永欣
LI Man;XIE Yongxin(Longyan Second Hospital,Fujian Province,Longyan 364000,China)
出处
《中国医学创新》
CAS
2021年第36期166-170,共5页
Medical Innovation of China
基金
龙岩市联合资金项目(2020LYF17052)。
