摘要
目的探讨连翘提取物对慢性盆腔炎模型大鼠的干预作用及对细胞间黏附分子-1(ICAM-1)、γ-干扰素(INF-γ)水平的影响。方法选取SPF级40只SD健康大鼠,按照双盲法随机分为正常组、模型组、阳性对照组、连翘提取物组,每组10只。模型组、阳性对照组、连翘提取物组建立慢性盆腔炎大鼠模型,建模型成功后,阳性对照组给予剂量为0.065 g/kg的氟哌酸胶囊剂灌胃处理,连翘提取物组使用连翘提取物250 mg/kg进行灌胃给药,正常组和模型组不作任何处理。对4组大鼠免疫球蛋白含量、ICAM-1、INF-γ、TLR4、NF-kB水平、子宫系数、红细胞压积、血浆粘度、右侧子宫质量及肿胀度进行检测。结果连翘提取物组大鼠血浆免疫球蛋白含量、INF-γ水平均高于阳性对照组,且ICAM-1、TLR4、NF-kB、子宫系数、红细胞压积、血浆粘度、子宫质量、肿胀度均低于阳性对照组,差异有统计学意义(均P<0.05)。结论连翘提取物能够有效缓解大鼠子宫黏连,改善体内炎症反应及血流病变异常,其机制可能与子宫组织ICAM-1、INF-γ表达有关。
Objective To study the effect of Forsythia suspensa extract on the level of ICAM-1(intercellular adhesion molecule-1)and inf-γ(interferon gamma)in chronic pelvic inflammatory model rats.Methods 40 SD healthy rats of SPF grade were randomly divided into normal group,model group,positive control group and Forsythia Extract group according to double blind.The model group,positive control group and Forsythia Extract group were used to establish the chronic pelvic inflammatory rat model.After the model was established successfully,the positive control group was given 0.065g/kg norfloxacin capsule,and the Forsythia Extract group was given 250mg/kg The normal group and the model group were not treated.The contents of immunoglobulin,ICAM-1,inf-γ,TLR4,NF-κB,uterine coefficient,hematocrit,plasma viscosity,right uterine mass and swelling degree were measured.Results The content of plasma immunoglobulin and inf-γwater in Forsythia suspense extract group were higher than those in the positive control group,and ICAM-1,TLR4,NF KB,uterine coefficient,hematocrit,plasma viscosity,uterine quality and swelling degree were lower than those in the positive control group(P<0.05).Conclusion Forsythia suspensa extract can effectively improve uterine adhesiveness,improve the inflammatory response and abnormal blood flow in rats.Its mechanism may be related to the expression of ICAM-1 and inf-γin uterine tissue.
作者
张永会
罗福兰
胡红娟
ZHANG Yonghui;LUO Fulan;HU Hongjuan(Department of Gynaecology, Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming 650021, China)
出处
《西部医学》
2022年第2期190-194,共5页
Medical Journal of West China
关键词
慢性盆腔炎
连翘提取物
细胞间黏附分子-1
Γ-干扰素
Chronic pelvic inflammatory disease
Forsythia suspensa extract
Intercellular adhesion molecule-1
Interferon gamma
