摘要
为探讨毫米波辐射对小鼠雄性生殖系统的损伤效应,将频率34.5 GHz、功率密度10 mW/cm^(2)的毫米波作为辐射源,以8周龄BALB/c雄性小鼠为实验对象,采用固定体位每天辐照2 h,持续9周。在辐照期间,每周进行苏木精-伊红染色法检测小鼠睾丸组织形态;在辐照第4~9周,每周通过原位末端转移酶标记技术染色检测睾丸细胞凋亡,小鼠尾静脉注射伊文思蓝检测血睾屏障受损情况,计算机辅助精子分析检测附睾精子活力。结果表明:在辐照4周内,小鼠各项指标均无异常;从辐照第5周开始,辐照组小鼠出现睾丸损伤,主要表现为生精上皮排列紊乱和凋亡增加、血睾屏障破坏、精子数量及活力下降,且随着时间的延长辐射损伤加重。本研究提示,长期的毫米波辐射对雄性小鼠存在生殖损伤效应,且损伤效应与辐照之间存在时效关系。
To investigate the detrimental effect of millimeter wave radiation on the reproductive system of male mice,millimeter waves with a frequency of 34.5 GHz and a power density of 10 mW/cm^(2) were used as radiation sources.Eight-week-old BALB/c male mice were irradiated for 2 h daily for 9 weeks in a fixed position.Hematoxylin and eosin staining of tissue sections was performed weekly during radiation to detect testicular histomorphology in mice.In situ terminal transferase labeling staining was performed weekly to detect testicular apoptosis at weeks 4 to 9 of radiation.Evans blue was injected into the tail vein of each mouse to detect blood-testis barrier damage.Computer-assisted sperm analysis was used to detect epididymal sperm motility.The results showed that there were no abnormalities in all indices of the mice within 4 weeks of exposure.From week 5 of exposure,testicular injury occurred in the irradiated mice and primarily manifested as disorganized spermatogenic epithelium,increased apoptosis,disruption of the blood-testis barrier,and decreased sperm count and motility.The injury was aggravated with further irradiation time.The data suggest that long-term millimeter wave radiation causes reproductive damage in male mice,with a time-effect relationship between the damage and radiation.
作者
唐正
魏金花
张磊
翟倩
袁建林
李臻
TANG Zheng;WEI Jinhua;ZHANG Lei;ZHAI Qian;YUAN Jianlin;LI Zhen(Department of Human Anatomy and Histoembryology,Fourth Military Medical University,Xi’an 710032,China;Department of Urology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China;Department of Obstetrics and Gynecology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China)
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2022年第1期48-55,共8页
Journal of Radiation Research and Radiation Processing
基金
空军军医大学军事医学“珠峰工程”(2018YYZT09)
西京医院学科助推计划(XJZT18D05)
陕西省重点研发计划(2021SF-055)资助。
关键词
毫米波辐射
雄性生殖
睾丸
凋亡
精子
Millimeter wave radiation
Male reproduction
Testis
Apoptosis
Sperm
