摘要
【目的】建立春尺蠖Apocheima cinerarius Erschoff转录组数据库,注释相关基因功能,挖掘其嗅觉相关基因,并初探其系统进化关系,为今后深入研究春尺蠖嗅觉感受机理奠定分子基础,从而运用相关基因进行有效防治提供分子信息学基础。【方法】采用新一代高通量测序平台(Illumina HiSeqTM 2500)对春尺蠖雌雄成虫进行转录组测序,经数据组装,筛选嗅觉相关基因,并采用聚类及功能注释对测序结果进行分析。【结果】从春尺蠖转录组数据库(https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA648143)基因数据中组装获得37 542条Unigenes,与公共数据库比对,共有17 296条Unigenes获得注释,与斜纹夜蛾Spodoptera litura相似基因数最多,达16.3%。GO数据库注释显示,11 174条Unigenes的分子功能分布于细胞组分、分子功能和生物过程3大类的55个功能组。KEGG数据库比对分析结果表明,5444个Unigenes形成了230个代谢通路。结合基因注释和blastp验证结果共获取嗅觉相关基因70个,包括22个气味结合蛋白(odorant binding proteins, OBPs)基因,23个化学感受蛋白(chemosensory proteins, CSPs)基因,14个气味受体(odorant receptor, OR)基因(包括13个普通受体和1个共受体ORco),8个离子型受体(Ionotropic receptors, IRs)基因,3个感觉神经元膜蛋白(sensory neuron membrane proteins, SNMPs)基因。与茶尺蠖Ectropis oblique、国槐尺蠖Semiothisa cinerearia、家蚕Bombyx mori、亚洲玉米螟Ostrinia furnacalis等4种昆虫的96个CSPs构建的系统进化树结果显示,春尺蠖与茶尺蠖形成了清晰的直系同源谱系,共有9对CSPs聚到同一分支上。【结论】本研究首次构建春尺蠖成虫转录组数据库,鉴定得到雌雄成虫嗅觉相关基因,并对鉴定出的基因进行系统进化探究,为春尺蠖的基因功能挖掘及嗅觉识别机理研究提供了分子基础数据。
【Objective】In this study, the transcriptome database of Apocheima cinerarius was established to annotate the functions of these genes and excavate olfactory related genes, aiming at exploring the phylogenetic relationship of olfactory sensory mechanism of A.cinerarius tolay a molecular foundation for further of olfactory sensory mechanism of A.cinerarius,and it will be provide molecular informatics foundation for effective prevention and control of the insect.【Method】With the aid of using new generation of high-throughput sequencing platform(Illumina HiSeqTM 2500) methords, we performed transcriptional profiling for male and female adults of A.cinerarius.After data assembly, olfactory related genes were screened, and the sequencing results were analyzed by genes clustering and functional annotation.【Result】37 542 Unigenes were assembled from the transcriptome database(https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA648143),and a total of 17 296 Unigenes were annotated by comparing the transcriptome data with the public database, these unigenes were 16.3% similar to Spodoptera litura which is the most alike.11 174 Unigenes were distributed in 55 functional groups in the GO database, including the three categories of cell components, molecular functions and biological processes.KEGG analysis results showed that a total of 5444 Unigenes formed 230 metabolic pathways.The combination of gene annotation information and blastp verified a total of 70 olfactory related genes, including 22 Odorant binding proteins(OBPs),23 Chemosensory proteins(CSPs),and 14 Odorant receptors(ORs)(including 13 Ordinary receptorsand 1 ORco),8 Ionotropic receptor(IRs)and 3 Sensory neuron membrane proteins(SNMPs).The phylogenetic tree of 96 CSPs constructed with Ectropis oblique,Semiothisa cinerearia,Bombyx mori,Ostrinia furnacalis and the other four insects showed that A.cinerarius and E.oblique formed a clear orthologous pedigree, and a total of 9 pairs of CSPs were clustered in the same phylogenetic treebranch.【Conclusion】In this study, the transcriptome data of A.cinerarius adult was established for the first time, olfactory-related genes were identified, and investigated the phylogenetic evolution of the identified genes.These results provided basic molecular for the function research and olfactory recognition mechanism of A.cinerarius.
作者
陈龙
单艳敏
廖桂堂
崔阔澍
伍亚琼
杨淞杰
CHEN Long;SHAN Yan-min;LIAO Gui-tang;CUI Kuo-shu;WU Ya-qiong;YANG Song-jie(Agriculture Department,Hetao College,Bayannaoer,Inner Mongolia 015000,China;Inner Mongolia Forestry and Grassland Pest Control and Quarantine Station,Huhehot 010051,China;Chengdu University of Information Technology,Chengdu 610225,China;Sichuan Agricultural Technology Extension Station,Chengdu 610041,China;Plant Protection Station of Sichuan Provincial Department of Agriculture and Rural Affairs,Chengdu 610041,China)
出处
《西南农业学报》
CSCD
北大核心
2022年第1期120-129,共10页
Southwest China Journal of Agricultural Sciences
基金
河套学院校级重点项目(HYZZ201927)
内蒙古自治区高等学校科学技术研究重点项目(NJZZ20220)。
关键词
春尺蠖
高通量测序
基因注释
嗅觉相关基因
Apocheima cinerarius
High-throughput sequencing
Gene annotation
Olfaction-related genes
