摘要
目的探讨脂肪酸结合蛋白4(FABP4)在同型半胱氨酸(Hcy)诱导的大鼠心肌细胞焦亡中的作用。方法培养大鼠心肌细胞,分为对照组(0 mmol/L Hcy)与Hcy组(3 mmol/L Hcy),采用Western blotting检测各组FABP4、焦亡相关蛋白NOD样受体蛋白3(NLRP3)、半胱天冬酶-1(caspase-1)、白细胞介素-1β(IL-1β)的表达情况;采用qRT-PCR检测FABP4 mRNA表达变化;采用免疫荧光染色法检测焦亡相关蛋白的表达变化;用FABP4抑制剂(BMS-309403)处理心肌细胞,然后将细胞分为对照组(0 mmol/L Hcy)、Hcy组(3 mmol/L Hcy)及Hcy+BMS-309403组(3 mmol/L Hcy和50μmol/L BMS-309403),再次检测焦亡相关蛋白的表达水平。结果 Western blotting检测结果显示,与对照组比较,Hcy组FABP4、NLRP3、caspase-1、IL-1β表达水平明显升高(P<0.05);qRT-PCR结果显示,与对照组比较,Hcy组FABP4表达水平明显升高(P<0.05);免疫荧光染色结果显示,与对照组比较,Hcy组NLRP3、caspase-1、IL-1β荧光强度明显增强。加入FABP4抑制剂(BMS-309403)同时给予Hcy干预后,与Hcy组比较,Hcy+BMS-309403组FABP4、NLRP3、caspase-1、IL-1β表达水平明显下降(P<0.05或P<0.01)。结论 FABP4可能对Hcy诱导的大鼠心肌细胞焦亡有促进作用。
Objective To investigate the effect of fatty acid binding protein 4(FABP4) in cardiomyocyte pyroptosis induced by homocysteine(Hcy). Methods The cultured rat cardiomyocytes were divided into two groups: control group(0 mmol/L Hcy) and Hcy group(3 mmol/L Hcy). The expression of FABP4 and pyroptosis related proteins [NOD-like receptor protein 3(NLRP3), caspase-1, interleukin-1β(IL-1β)] were detected by Western blotting. The expression level of FABP4 mRNA was detected by qRT-PCR. The changes of expression of pyroptosis associated proteins were detected by immunofluorescence staining. The cultured cardiomyocytes were treated with FABP4 inhibitor(BMS-309403), and then divided into control group(0 mmol/L Hcy), Hcy group(3 mmol/L Hcy) and Hcy+BMS-309403 group(3 mmol/L Hcy and 50 μmol/L BMS-309403), and the expression levels of pyroptosis related proteins were then re-detected. Results Compared with control group, Western blotting showed that the expression levels of FABP4, NLRP3, caspase-1 and IL-1β in Hcy group increased significantly(P<0.05);Compared with control group, qRT-PCR results showed that the expression of FABP4 in Hcy group increased significantly(P<0.05);Compared with control group, immunofluorescence staining results showed that the fluorescence intensity of NLRP3, caspase-1 and IL-1β in Hcy group increased significantly. After adding FABP4 inhibitor(BMS-309403) and Hcy treatment,the expression levels of FABP4, NLRP3, caspase-1 and IL-1β in Hcy+BMS-309403 group decreased significantly compared with Hcy group(P<0.05 or P<0.01). Conclusion FABP4 may promote cardiomyocytes pyroptosis induced by homocysteine.
作者
董小艳
刘达越
徐灵博
顾铃毓
马胜超
杨安宁
杨勇
刘云
姜怡邓
Dong Xiao-Yan;Liu Da-Yue;Xu Ling-Bo;Gu Ling-Yu;Ma Sheng-Chao;Yang An-Ning;Yang Yong;Liu Yun;Jiang Yi-Deng(School of Basic Medical Sciences,Ningxia Medical University,Yinchuan 750004,China;NHC Key Laboratory of Metabolic Cardiovascular Diseases Research,Yinchuan 750004,China;Ningxia Key Lab of Vascular Injury and Repair Research,Yinchuan 750004,China;Clinical Medical College,Ningxia Medical University,Yinchuan 750004,China;Department of Nuclear Medicine,People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750004,China;Department of Radiology,General Hospital of Ningxia Medical University,Yinchuan 750004,China)
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2022年第1期33-38,共6页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金地区科学基金(81860044)
国家自然科学基金青年科学基金(81900273)
宁夏回族自治区重点研发计划重点项目(2020BFH02003)
宁夏自然科学基金(NZ14151)。
