摘要
目的探究麻醉对肺缺血再灌注损伤(lung ischemia-reperfusion injury,LIRI)大鼠所致术后认知功能障碍(post operative cognitive dysfunction,POCD)的影响,从而为临床麻醉药物的实施提供实验和理论依据。方法建立成年大鼠肺缺血再灌注动物模型,选用成年雄性大鼠共30只,初始体重控制在250~320 g左右,随机分为3组,每组10只。假手术组(S组):仅打开胸腔,分离左肺门和肺动静脉,但不阻断;肺缺血再灌注损伤组(I/R组):肺缺血1 h后再灌注2 h[1];七氟烷预处理组(SP组):吸入浓度为2.1%的七氟烷30 min后制备肺缺血再灌注模型。动物模型制备完成后,通过Morris水迷宫实验分别测定每组大鼠术后学习及记忆能力的变化[2];上述实验结束后处死大鼠,取大鼠海马组织和肺组织,检测髓样细胞触发受体2(triggering receptor expressed on myeloid cells 2,TREM2)[3]和糖原合成激酶-3β(gylcogen synthase kinase 3β,GSK-3β)的表达。结果Morris水迷宫实验表明,经七氟烷预处理后LIRI损伤大鼠较I/R组逃避潜伏期有明显缩短,穿台次数明显增多,且运动轨迹相对集中,表明七氟烷预处理可在一定程度上改善大鼠的记忆损伤。Western Blot结果表明,与I/R组相比,经七氟烷预处理后TREM2和GSK-3β都有所上调,提示七氟烷预处理可能通过上调TREM2和GSK-3β的表达改善大鼠的认知功能损害。结论七氟烷可以改善肺缺血再灌注损伤大鼠的认知功能,为临床研究奠定了实验基础。
Objective To investigate the effects of anesthesia on post-operative cognitive dysfunction(POCD)induced by lung ischemia-reperfusion injury(LIRI)in rats to provide experimental and theoretical basis for the application of clinical anesthetic drugs.Methods An adult rat model of lung ischemia-reperfusion was established.30 adult male rats were randomly divided into three groups(10 rats in each group),with the initial weight of 250~320 g.Sham Operation Group(Group S):only the chest was opened,the left hilum and pulmonary artery and vein were separated but not blocked;Lung ischemia-reperfusion injury group(I/R group):lung reperfusion 2 h after 1 h of ischemia[1].Sevoflurane preconditioning group(SP group):sevoflurane with 2.1%concentration was inhaled for 30 min to establish the lung ischemia-reperfusion model.After the establishment of the animal model,the changes of the learning and memory ability of the rats in each group were measured by Morris water maze experiment.[2]At the end of the above experiment,the rats were sacrificed,and the hippocampal tissue and lung tissue of the rats were collected.The expressions of triggering receptor expressed on myeloid cells 2(TREM2)and glycogen synthase Kinase 3β(GSK-3β)were detected.Results Morris water maze test showed that compared with the I/R group,sevoflurane pretreatment significantly shortened the escape latency of LIRI injured rats,significantly increased the number of crossing the stage,and relatively concentrated the movement trajectory,suggesting that sevoflurane pretreatment can improve the memory impairment of rats to a certain extent.Western Blot results showed that both TREM2 and GSK-3βwere upregulated after sevoflurane pretreatment compared with the I/R group,suggesting that sevoflurane pretreatment may ameliorate cognitive impairment in rats by up-regulating TREM2 and GSK-3βexpression.Conclusion Sevoflurane can effectively improve the postoperative cognitive function of rats with lung ischemia-reperfusion injury,which has laid the experimental foundation for clinical research.
作者
夏若馨
刘天豪
张从利
张引
刘刚
李晓红
张阳
Xia Ruoxin;Liu Tianhao;Zhang Congli;Zhang Yin;Liu Gang;Li Xiaohong;Zhang Yang(Bengbu Medical College;Department of Anesthesiology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000 China)
出处
《锦州医科大学学报》
2022年第1期21-25,共5页
Journal of Jinzhou Medical University
基金
2020年省级大学生创新创业训练计划项目,项目编号:S202010367115。
关键词
肺缺血再灌注损伤
麻醉
认知功能障碍
lung ischemia-reperfusion injury
anesthesia
cognitive impairment
