摘要
目的应用四种方法分别进行血小板计数,旨在为实验室检测血小板计数异常时推荐适宜的复检方法。方法收集2020年8-9月在连云港市第二人民医院住院治疗的患者300例为研究对象,依据电阻抗法的血小板计数结果将研究对象分为低值组、中值组、高值组各100例,并基于流式细胞术作为血小板计数的参考方法,分别与电阻抗法、牛鲍计数板法和改良血涂片法的计数结果进行一致性分析。结果电阻抗法[低值组:(86.1±10.3)×10^(9)/L,中值组:(221.8±41.8)×10^(9)/L,高值组:(441.3±51.4)×10^(9)/L]、牛鲍计数板法[低值组:(85.2±10.1)×10^(9)/L,中值组:(219.3±37.7)×10^(9)/L,高值组:(443.1±54.5)×10^(9)/L]、改良血涂片法[低值组:(86.1±10.1)×10^(9)/L,中值组:(218.1±37.7)×10^(9)/L,高值组:(442.6±53.3)×10^(9)/L]的血小板计数结果与流式细胞术[低值组:(85.4±10.1)×10^(9)/L,中值组:(220.7±42.0)×10^(9)/L,高值组:(440.9±50.0)×10^(9)/L]的血小板计数结果差异均无统计学意义(均P>0.05);Bland-Altman分析显示,上述三种方法与流式细胞术的血小板计数结果之间均具有一致性。结论改良血涂片法与参考方法的比对结果一致,且具有不需要特殊仪器、计数同时可以观察血细胞形态、涂片易于长期保存等优点,建议首选改良血涂片法作为血小板计数的复检方法。
Objective Four methods were used to count platelets to recommend suitable retest methods for abnormal blood platelet count.Methods A total of 300 patients who received treatment in the Second People's Hospital of Lianyungang during August-September 2020 were included in this study.They were divided into low-value,median-value,and high-value groups(n=100/group)according to blood platelet counts determined by the electrical impedance method.The consistency in blood platelet counting was analyzed between flow cytometry and electrical impedance method,Neubauer chamber method,and modified blood smear method.Results There was no significant difference in blood platelet count between the electrical impedance method[low-value group:(86.1±10.3)×10^(9)/L,median-value group:(221.8±41.8)×10^(9)/L,high-value group:(441.3±51.4)×10^(9)/L,Neubauer chamber method[low-value group:(85.2±10.1)×10^(9)/L,median-value group:(219.3±37.7)×10^(9)/L,high-value group:(443.1±54.5)×10^(9)/L,modified blood smear technique[low-value group:(86.1±10.1)×10^(9)/L,median-value group:(218.1±37.7)×10^(9)/L,high-value group:(442.6±53.3)×10^(9)/L],and flow cytometry[low-value group:(85.4±10.1)×10^(9)/L,median-value group:(220.7±42.0)×10^(9)/L,high-value group:(440.9±50.0)×10^(9)/L](all P>0.05).The Bland-Altman analysis revealed that the electrical impedance method,Neubauer chamber method and modified blood smear method,and flow cytometry showed consistency in blood platelet count.Conclusion The modified blood smear method showed consistency with the electrical impedance method,Neubauer chamber method,and flow cytometry in blood platelet counting.It does not require a special instrument and can help observe cell morphology for blood platelet counting.In addition,the blood smears are easy to be preserved.The modified blood smear technique should be the first choice for re-checking blood platelet counts.
作者
刘倩
李欢
尹笑笑
纵如原
杨伏猛
Liu Qian;Li Huan;Yin Xiaoxiao;Zong Ruyuan;Yang Fumeng(Department of Laboratory Medicine,the Second People's Hospital of Lianyungang,Lianyungang 222006,Jiangsu Province,China)
出处
《中国基层医药》
CAS
2022年第2期229-234,共6页
Chinese Journal of Primary Medicine and Pharmacy
基金
江苏省卫生健康委员会医学科研面上项目(M2020079)
南京医科大学康达学院科研发展基金重点项目(KD2019KYJJZD011)
蚌埠医学院自然科学基金重点项目(BYKY18178,2020byzd342)。
关键词
实验室技术和方法
血小板
血小板计数
电阻抗
血涂片
流式细胞术
牛鲍计数板法
Laboratory techniques and procedures
Blood platelets
Platelet count
Electric impedance
Blood smear
Flow cytometry
Neubauer hemocytometer method
