摘要
肉类掺假现象普遍存在,导致严重的公共卫生风险和侵犯宗教信仰行为。快速、有效、准确和可靠的检测技术是有效监管肉类掺假的关键手段。近年来,基于高通量测序的DNA宏条形码技术发展迅速,具有高通量、高精度和速度快等特点,并且可以实现复杂样品中多个物种的同时检测,因而在肉类及其制品的掺假检测方面具有明显的优势。文中介绍了近20年来高通量测序技术的主要发展历程、DNA宏条形码的技术特点和研究方法,综述了近年来DNA宏条形码技术在肉类掺假检测中的应用,讨论分析了DNA宏条形码技术应用于肉类掺假检测领域面临的挑战,并对该技术的未来发展趋势进行了展望。
Adulteration in meat products is a widespread issue that could lead to serious threats to public health and religious violations.Technology that offers rapid,sensitive,accurate and reliable detection of meat species is the key to an effectual monitoring and control against meat adulteration.In recent years,high-throughput sequencing-based DNA metabarcoding technology has developed rapidly.With the characteristics of being high-throughput,highly precise and high-speed,this technology can simultaneously identify multiple species in complex samples,thus offering pronounced advantages in the surveillance of adulteration in meat and meat products.Starting with an introduction of the major developments in the high-throughput sequencing technology in the past two decades,this review provides an overview of the technical characteristics and research methods of DNA metabarcoding,summarizes the application of DNA metabarcoding technology in meat adulteration detection over the last few years,discusses the challenges of using DNA metabarcoding technology in the detection of meat adulteration,and provides future prospects on the development of this technology.
作者
甘永琦
卢曼曼
赖青鸟
朱斌
GAN Yongqi;LU Manman;LAI Qingniao;ZHU Bin(Guangxi Institute for Food and Drug Control,Nanning 530021,Guangxi,China;Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530004,Guangxi,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2022年第2期411-426,共16页
Chinese Journal of Biotechnology
基金
广西壮族自治区自然科学青年基金(2018JJB130010)。
关键词
高通量测序
DNA条形码
宏条形码技术
肉类掺假
物种鉴定
食品安全
high-throughput sequencing
DNA barcoding
metabarcoding technology
meat adulteration
species identification
food safety
