摘要
株型是由多个形态和生理性状集成的复合性状,它与水稻产量密切相关。挖掘优异株型等位基因或QTL,对水稻超高产育种具有重要意义。本研究利用籼稻昌恢121和粳稻Koshihikari构建的208个染色体片段置换系(chromosome segment substitution lines,CSSLs),在3个环境下,对控制株高、剑叶形态和分蘖数的QTL进行检测,共鉴定到35个株型性状QTL,分布于11条染色体上(除9号染色体以外),解释表型变异2.00%~22.86%。值得关注的是qPH-1-1、qFLW-6和qFLA-3均能在3个环境下被检测到,其中qFLW-6为1个新鉴定到的剑叶宽QTL。对qPH-1-1和qFLA-3位点进行鉴定,验证了这2个位点等位基因的加性效应和环境稳定性。本研究为株型性状QTL的进一步精细定位、克隆及分子辅助聚合育种奠定了基础。
Plant architecture is a compound trait integrated with multiple morphological and physiological traits,and it is closely related to rice yield.Deciphering excellent plant architecture alleles or QTLs is of great significance for high-yield rice breeding.In this study,we constructed a set of Changhui 121/Koshihikari chromosome segment substitution lines(CSSLs)with the size of 208 in our laboratory.QTLs controlling plant height,flag leaf morphology,and tiller numbers were detected under three environments.A total of 35 QTLs for rice architecture were identified on 11 chromosomes except chromosome 9,and the range of the phenotypic variation explaining was 2.00%–22.86%.It was worth noting that qPH-1-1,qFLW-6,and qFLA-3 could be detected in three environments,among which qFLW-6 was a newly identified QTL of the flag leaf width.Phenotypic identification verified that the additive effects and environmental stability of the two locus alleles by the replacement lines carrying qPH-1-1 and sites.The results of this study laid the foundation for further fine mapping and cloning of QTLs for rice plant architecture and the molecular marker-assisted selection(MAS)in rice breeding.
作者
王小雷
李炜星
欧阳林娟
徐杰
陈小荣
边建民
胡丽芳
彭小松
贺晓鹏
傅军如
周大虎
贺浩华
孙晓棠
朱昌兰
WANG Xiao-Lei;LI Wei-Xing;OU-YANG Lin-Juan;XU Jie;CHEN Xiao-Rong;BIAN Jian-Min;HU Li-Fang;PENG Xiao-Song;HE Xiao-Peng;FU Jun-Ru;ZHOU Da-Hu;HE Hao-Hua;SUN Xiao-Tang;ZHU Chang-Lan(Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education/College of Agronomy,Jiangxi Agricultural University/Research Center of Super Rice,Engineering and Technology,Nanchang 330045,Jiangxi,China)
出处
《作物学报》
CAS
CSCD
北大核心
2022年第5期1141-1151,共11页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31860373)
江西省“5511”优势科技创新团队项目(20165BCB19005)资助。
关键词
水稻
染色体片段置换系
株型
数量性状基因座位
rice
chromosome segment substitution lines
plant architecture
quantitative trait locus
