摘要
通过陕西省绞股蓝总黄酮对ABTS、超氧阴离子及DPPH自由基的清除能力来评价其抗氧化性能,并采用半数稀释法测定绞股蓝总黄酮对金黄色葡萄球菌、大肠杆菌、粪链球菌、枯草芽孢杆菌和普通变形杆菌等5种常见致病菌的最低抑制浓度。结果表明,绞股蓝总黄酮对ABTS、超氧阴离子及DPPH自由基均具有一定的清除能力,且对超氧阴离子及DPPH自由基清除能力较强;绞股蓝总黄酮对金黄色葡萄球菌等5种致病菌均有一定的抑制作用,其中对大肠杆菌和金黄色葡萄球菌抑制作用较强。
The antioxidant activity of total flavonoids from Gynostemma pentaphyllum(Thunb.)Makino in Shaanxi province was evalu⁃ated by scavenging ABTS,superoxide anion and DPPH free radical.The minimum inhibitory concentration of total flavonoids from Gy⁃nostemma pentaphyllum(Thunb.)Makino against Staphylococcus aureus,Escherichia coli,Streptococcus faecalis,Bacillus subtilis and Proteus vulgaris were determined by half dilution method.The results showed that the total flavonoids extracted from Gynostemma pen⁃taphyllum(Thunb.)Makino had certain scavenging ability in ABTS,superoxide anion and DPPH free radicals.In particular,it had strong scavenging ability in superoxide anion and DPPH free radicals.It had a certain inhibitory effect on 5 kinds of pathogenic bacte⁃ria,especially Escherichia coli and Staphylococcus aureus.
作者
马泽刚
辛超
杨新春
吴秀丽
徐静
MA Ze-gang;XIN Chao;YANG Xin-chun;WU Xiu-li;XU Jing(College of Education and Physical Education,Sichuan Vocational College of Health and Rehabilitation,Zigong 643000,Sichuan,China;School of Chemical Engineering and Chemistry,Harbin Institute of Technology,Harbin 150001,China)
出处
《湖北农业科学》
2022年第4期150-153,共4页
Hubei Agricultural Sciences
基金
四川省教育厅自然科学基金项目(17ZB0366)
自贡市重点科技计划项目(2020YGJC30)
四川卫生康复职业学院校级重点项目(CWKY-2021Z-03)。
关键词
绞股蓝
总黄酮
抗氧化
抑菌
Gynostemma pentaphyllum(Thunb.)Makino
total flavonoids
antioxidant
bacteriostasis