摘要
为探究拉氏[鱼岁]精子的生理特性和提高拉氏[鱼岁]人工繁殖受精率,探明金属离子浓度、温度、pH等因子变化对拉氏[鱼岁]精子活力的影响。取10尾拉氏[鱼岁]雄亲鱼(体质量80.0~100.0 g,体长10.0~20.0 cm),测定其在不同离子[Na^(+)、K^(+)、Ca^(2+)、葡萄糖、Tris缓冲剂(C_(4)H_(11)NO_(3)]、不同温度[(8.0±1.0)℃、(12.0±1.0)℃、(16.0±1.0)℃、(20.0±1.0)℃、(24.0±1.0)℃、(30.0±1.0)℃]、不同pH(5.0、6.0、7.0、8.0、9.0)下的精子快速运动时间和寿命。试验结果表明,Na^(+)浓度为100 mmol/L时,拉氏[鱼岁]精子活力最强,精子快速运动时间为(72.05±9.93)s,其上升率为28.1%,寿命为(106.86±6.00)s,上升率为39.4%;K^(+)浓度为75 mmol/L时,精子活力最强,快速运动时间为(59.47±5.76)s,上升率为5.7%,寿命为(252.11±218.64)s,上升率为228.9%;葡萄糖浓度为0~100 mmol/L时,精子快速运动时间无显著差异,但寿命随葡萄糖浓度的增加而增加;Ca^(2+)和Tris缓冲剂对拉氏[鱼岁]精子活力具有明显抑制作用,快速运动时间和寿命均出现缩短。水温在(20.0±1.0)℃时,拉氏[鱼岁]精子活力最强,精子快速运动时间和寿命分别为(32.99±7.50)s、(78.77±8.47)s;pH 8.0时拉氏[鱼岁]精子活力最强,精子快速运动时间、寿命分别为(45.42±6.56)s、(475.02±46.35)s。复合液[V(Na^(+)100 mmol/L)∶V(K^(+)75 mmol/L)=1∶1]对精子快速运动时间无显著差异,而寿命极显著高于其他各条件,达(745.67±273.08)s,上升率为968.6%。研究结果表明,适宜Na^(+)、K^(+)、葡萄糖对拉氏[鱼岁]精子活力有促进作用,Ca^(2+)、Tris缓冲剂对拉氏[鱼岁]精子活力有抑制效果。水温(20.0±1.0)℃,pH 7.0~8.0时,拉氏[鱼岁]精子活力最佳。复合液[V(Na^(+)100 mmol/L)∶V(K^(+)75 mmol/L)=1∶1]极显著提高精子寿命。
In order to understand the physiological characteristics of sperm of fat minnow Phoxinus lagowskii and improve its fertilization rate,we studied the effects of metal ion concentrations,temperature and pH on the sperm motility of fat minnow.The sperm fast moving time(FT)and life time(LT)were measured of 10 individuals of male fat minnow(body weight of 80.0—100.0 g and body length of 10.0—20.0 cm)under different ions[Na^(+),K^(+),Ca^(2+),glucose,and Tris buffer(C_(4)H_(11)NO_(3))],different temperatures[(8.0±1.0)℃,(12.0±1.0)℃,(16.0±1.0)℃,(20.0±1.0)℃,(24.0±1.0)℃,and(30.0±1.0)℃]and different pH(5.0,6.0,7.0,8.0,and 9.0).The results showed that the sperm had the FT of(72.05±9.93)s and rising rate of 28.1%,the LT of(106.86±6.00)s with rising rate of 39.4%at Na^(+)concentration of 100 mmol/L.The sperm motility was the strongest at the K^(+)concentration of 75 mmol/L,and had the FT of(59.47±5.76)s with rising rate of 5.7%,and the LT of(252.11±218.64)s with rising rate of 228.9%,without no significant difference in the FT between 0 and 100 mmol/L glucose concentrations,but increase in LT with the increase in glucose concentration.In addition,Ca^(2+)and Tris buffer inhibited the sperm motility,with short FT and LT.The sperm motility was better with FT of(32.99±7.50)s and LT of(78.77±8.47)s at temperature of(20.0±1.0)℃.The longest FT and LT was observed at pH 8.0,(45.42±6.56)s and(475.02±46.35)s.Without significant difference in the time of sperm rapid movement(745.67±273.08)s in compound solution B[V(Na^(+)100 mmol/L)∶V(K^(+)75 mmol/L)=1∶1],significantly higher life time than that in other conditions,with an increase rate of 968.6%.It was found that appropriate Na^(+),K^(+)and glucose concentration promoted the sperm motility of fat minnow,Ca^(2+)and that Tris buffer inhibited the sperm motility.The best sperm motility was observed in the fat minnow exosed to water temperature of(20.0±1.0)℃and pH range of 7.0—8.0.Compound solution B[V(Na^(+)100 mmol/L)∶V(K^(+)75 mmol/L)=1∶1]led to significant increase in sperm longevity.
作者
吴晨
骆小年
段友健
曾永聪
缪云亮
韩峰
罗超
WU Chen;LUO Xiaonian;DUAN Youjian;ZENG Yongcong;MIAO Yunliang;HAN Feng;LUO Chao(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)
出处
《水产科学》
CAS
CSCD
北大核心
2022年第2期210-217,共8页
Fisheries Science
基金
辽宁省“百千万人才工程”项目(793018201)
大连市科技创新基金资助项目(2018J12SN068)
大连海洋大学引进人才启动及在职培养博士启动项目(HDYJ201802)。
关键词
拉氏[鱼岁]
精子活力
离子浓度
温度
PH
Phoxinus lagowskii
sperm motility
ion concentration
temperature
pH
