摘要
目的:观察诺丽对氢化可的松致肾阳虚模型小鼠的影响,研究诺丽补肾改善记忆的功效,探讨其作用机制。方法:60只昆明小鼠随机分为对照组,模型组,人参组,诺丽高、中、低剂量组,每组10只,除对照组,其他组均灌胃氢化可的松建立肾阳虚模型,观察小鼠给药后记忆行为学变化,尼氏染色观察脑组织海马CA3区锥体细胞形态和尼氏体数量病理改变,ELISA检测小鼠脑组织神经递质、cAMP、cGMP及PKA含量变化。结果:与对照组相比,模型组跳台实验和避暗实验潜伏期明显缩短(P<0.01),错误次数明显增多(P<0.01),消退潜伏期明显缩短(P<0.01),消退错误次数明显增多(P<0.01),大脑海马CA3区尼氏体数量较少(P<0.01),脑组织神经递质5-HT、ACh、DA、NA表达降低(P<0.01),cAMP、PKA含量下降(P<0.01,P<0.05),cAMP/cGMP比值降低(P<0.05)。与模型组相比,人参和诺丽高、中剂量能明显延长小鼠跳台实验和避暗实验的潜伏期时间(P<0.01,P<0.05),降低错误次数(P<0.01,P<0.05),延长消退潜伏期时间(P<0.01,P<0.05),降低消退错误次数(P<0.01,P<0.05),增加大脑海马CA3区尼氏体数量(P<0.01),增加脑组织神经递质ACh、5-HT、DA、NA表达(P<0.01,P<0.05),升高cAMP、PKA含量(P<0.01),升高cAMP/cGMP比值(P<0.01)。结论:诺丽对氢化可的松致肾阳虚小鼠的记忆损伤有保护作用,其补肾改善记忆是通过调节cAMP/PKA信号通路蛋白,影响下游神经递质,促进神经元蛋白合成实现的。
Objective:To observe the effect of Noni on the model mice of Kidney-Yang deficiency induced by Hydrocortisone,and to explore its mechanism in improving memory.Methods:60 Kunming mice were randomly divided into the control group,the model group,the Ginseng group,the high-dose Noni group,the medium-dose Noni group and the low-dose Noni group,with 10 mice in each group.The model of Kidney-Yang deficiency was established by the administration of Hydrocortisone.The memory changes of mice were observed after the adiminstration.The number of pyramidal cells and Nissl’s bodies were observed by Nissl staining.The contents of cAMP,cGMP,PKA and neurotransmitters in the brain of mice were detected by ELISA.Results:Compared with those in the control group,the incubation periods of step-down test and passive avoidance test were significantly prolonged(P<0.01),the number of errors was significantly increased(P<0.01),the vanish incubation period was significantly shortened(P<0.01),the number of vanish errors was significantly increased(P<0.01),the number of Nissl’s bodies in CA3 area of hippocampus was less(P<0.01),the expressions of neurotransmitters of 5-HT,ACh,DA and NA decreased(P<0.01),the contents of cAMP and PKA decreased(P<0.05,P<0.01),and the ratio of cAMP/cGMP reduced in the model group(P<0.05).Compared with those in the model group,the incubation periods of step-down test and passive avoidance test were significantly prolonged(P<0.05,P<0.01),the number of errors was reduced(P<0.05,P<0.01),the time of vanish incubation period was prologned(P<0.05,P<0.01),the number of vanish errors was decreased(P<0.05,P<0.01),the number of Nissl bodies in CA3 area of hippocampus was increased(P<0.01),the expressions of neurotransmitters of 5-HT,ACh,DA and NA in brain tissue were increased(P<0.05,P<0.01),the contents of cAMP and PKA were increased(P<0.01),and the ratio of cAMP/cGMP was increased as well in the Genseng group and the Noni groups of high-dose and mediudm-dose(P<0.01).Conclusion:Noni has a protective effect on the memory impairment of Kidney-Yang deficiency mice model induced by Hydrocortisone.Its effects of tonifying Kidney and improving memory are achieved by regulating proteins of cAMP/PKA signaling pathway,influencing downstream neurotransmitters and promoting protein synthesis of neurons.
作者
张睿
邸松蕊
余淑惠
刘金莲
汤如莹
葛东宇
董瑞娟
张建军
ZHANG Rui;DI Songrui;YU Shuhui;LIU Jinlian;TANG Ruying;GE Dongyu;DONG Ruijuan;ZHANG Jianjun(Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中医药学报》
CAS
2022年第3期17-22,共6页
Acta Chinese Medicine and Pharmacology
基金
国家重点研发计划中医药现代化研究(2018YFC1706800)。
