摘要
目的观察脂氧素A_(4)(LXA_(4))对大鼠大脑中动脉闭塞再灌注(MCAO/R)模型缺血脑组织周边区半胱氨酸天冬氨酸蛋白水解酶1(Caspase-1)活性的影响。方法随机将SD雄性大鼠分为假手术组、模型对照组和LXA_(4)组,每组8只。模型对照组和LXA_(4)组采用线栓法制作MCAO/R模型,LXA_(4)组同时侧脑室注射LXA_(4)1 nmol。观察3组大鼠脑梗死体积、神经行为学评分、Caspase-1蛋白表达和活力、白细胞介素-1β(IL-1β)和IL-18浓度。结果与模型对照组比较,LXA_(4)组大鼠脑梗死体积显著缩小(P<0.01),神经行为学评分显著降低(P<0.01);大鼠缺血脑皮层周边区Caspase-1蛋白表达和活力显著减少(P<0.01);缺血脑皮层IL-1β和IL-18的浓度显著降低(P<0.05)。结论LXA_(4)抑制Caspase-1活性是其抗脑缺血-再灌注损伤的机制之一。
Objective To observe the effects of LXA_(4)(Lipoxin A_(4))on the activity of cysteinyl aspartate-specific proteases 1(Caspase-1)in the peripheral area of the ischemic cortex tissue in rats with middle cerebral artery occlusion/reperfusion(MCAO/R).Methods Adult male Sprague–Dawley rats were randomly divided into sham group,model control group,and LXA_(4)group,8 rats in each group.For rats of model control group and LXA_(4)group,the model rats of cerebral ischemia were made by occlusion of middle cerebral artery using an intraluminal filament method,and rats in LXA_(4)group were injected 1 nmol of LXA_(4).The infarct volume,the neurological deficit scores,the activity of Caspase-1,Caspase-1 protein expression,concentrations of interleukin-1β(IL-1β)and interleukin-18(IL-18)in three groups were observed.Results LXA_(4)effectively reduced infarct volume and neurological scores in MCAO/R rat(P<0.01).The activity and protein expression of Caspase-1 were significantly reduced by LXA_(4).(P<0.01)The concentrations of IL-1βand IL-18 were also significantly inhibited by LXA_(4)(P<0.01).Conclusion These results suggest that the neuroprotective effects of LXA_(4)were likely achieved by inhibiting the activity of Caspase-1.
作者
吴乐
武强
陈勇
WU Le;WU Qiang;CHEN Yong(Department of Neurology,Taikang Tongji(Wuhan)Hospital,Wuhan 430050,China;Department of Emergency Medicine,Taikang Tongji(Wuhan)Hospital,Wuhan 430050,China)
出处
《医药导报》
CAS
北大核心
2022年第2期175-179,共5页
Herald of Medicine
基金
2015年武汉市中青年医学骨干人才培养工程。
