摘要
目的研究口腔扁平苔藓(OLP)患者外周血淋巴细胞亚群、免疫球蛋白及补体水平变化情况,探讨免疫因素与OLP的关系及其临床意义。方法选取2019年10月至2020年9月宁夏医科大学总医院的100例OLP患者作为OLP组,同期体检的100例健康人群作为对照组。采用流式细胞术检测细胞免疫(淋巴细胞亚群CD3^(+)T细胞、CD4^(+)T细胞、CD3^(+)CD8^(+)T细胞、CD16^(+)CD56^(+)自然杀伤细胞、CD3^(-)CD19^(+)B细胞、CD4^(+)/CD8^(+))水平,采用免疫散射比浊法检测体液免疫(免疫球蛋白IgG、IgA、IgM及补体C3、C4)水平。结果OLP组CD3^(+)CD8^(+)T细胞水平低于对照组,CD4^(+)/CD8^(+)水平高于对照组,差异均有统计学意义(P<0.05);OLP组免疫球蛋白IgM水平低于对照组,差异有统计学意义(P<0.05)。结论OLP患者细胞免疫与体液免疫均存在一定的失衡。
Objective To study the changes of peripheral blood lymphocyte subsets,immunoglobulin and complement levels in patients with oral lichen planus(OLP),and to explore the relationship between immune factors and OLP and its clinical significance.Methods A total of 100 OLP patients from the General Hospital of Ningxia Medical University from October 2019 to September 2020 were collected as the OLP group,and 100 healthy people who underwent physical examination during the same period were collected as the control group.The level of cellular immunity(lymphocyte subsets CD3^(+)T cell,CD4^(+)T cell,CD3^(+)CD8^(+)T cell,CD16^(+)CD56^(+)natural killer cell,CD3^(-)CD19^(+)B cell and CD4^(+)/CD8^(+))were detected by flow cytometry.The level of humoral immunity(immunoglobulin IgG,IgA,IgM and complement C3,C4)were detected by immunoturbidimetry.Results The level of CD3^(+)CD8^(+)T cell in the OLP group was lower than that in the control group,and the level of CD4^(+)/CD8^(+)was higher than that in the control group,the differences were statistically significant(P<0.05).The level of immunoglobulin IgM in the OLP group was lower than that in the control group,and the difference was statistically significant(P<0.05).Conclusion There is a certain imbalance between cellular immunity and humoral immunity in OLP patients.
作者
师志云
李海波
潘琳
李莉
陈静
张玉英
李霞
李想
丁淑琴
王利茹
SHI Zhiyun;LI Haibo;PAN Lin;LI Li;CHEN Jing;ZHANG Yuying;LI Xia;LI Xiang;DING Shuqin;WANG Liru(Medical Experimental Center,General Hospital of Ningxia Medical University,Yinchuan,Ningxia 750004,China;Department of Clinical Laboratory,Ningxia Hui Autonomous Region People′s Hospital,Yinchuan,Ningxia 750002,China;Department of Laboratory Science,Clinical College,Ningxia Medical University,Yinchuan,Ningxia 750004,China)
出处
《检验医学与临床》
CAS
2022年第6期734-736,共3页
Laboratory Medicine and Clinic
基金
宁夏自然科学基金项目(2019AAC03216,2021AAC03326)
宁夏高等学校一流学科建设(宁夏医科大学国内一流建设学科临床医学)资助项目(NXYLXK2017A05)
2019年大学生创新创业训练计划项目(19-2010)。
