摘要
背景:Sam68基因表达失控会诱导多种类型癌细胞的细胞周期进程、细胞增殖、转化以及肿瘤发生和转移。然而,目前尚不确定Sam68在胃癌细胞中的具体作用和分子机制。目的:探讨Sam68表达与胃癌患者临床预后以及胃癌细胞生物学行为之间的相关性。方法:收集161例胃癌组织和癌旁正常组织,采用蛋白质印迹法和免疫组化染色检测Sam68表达,并分析其表达与胃癌患者临床病理特征之间的关系。以siRNA Sam68转染胃癌AGS细胞,CCK-8法检测细胞增殖,划痕实验评估细胞迁移能力,Transwell实验评估细胞侵袭能力。结果:Sam68在胃癌组织中的表达增加,并与浸润深度、TNM分期和淋巴结转移相关。Sam68高表达与胃癌患者的预后不良有关。转染Sam68 siRNA后,胃癌细胞增殖、细胞周期进程、迁移和侵袭能力均下降。结论:Sam68可能是一个新的评估胃癌细胞生长、迁移和侵袭的因子,对胃癌患者预后具有重要意义。
Background:The dysregulation of expression of Sam68 gene can induce cell cycle progression,cell proliferation,transformation,tumorigenesis,and metastasis of multiple types of cancer cells.However,the specific role and molecular mechanism of Sam68 in gastric cancer cells are still uncertain.Aims:To investigate the correlation of expression of Sam68 with prognosis of gastric cancer patients and biological behavior of gastric cancer cells.Methods:A total of 161 gastric cancer tissue and paracancerous normal tissue specimens were collected.Western blotting and immunohistochemical staining were used to detect the expression of Sam68,and its correlations with clinicopathological features were analyzed.Gastric cancer AGS cells were transfected by siRNA Sam68.Cell proliferation was evaluated by CCK-8 assay,cell migration ability was evaluated by scratch test,and cell invasion ability was evaluated by Transwell test.Results:Expression of Sam68 was increased in gastric cancer tissue,and was correlated with depth of infiltration,TNM staging and lymph node metastasis.The high expression of Sam68 was correlated with poor prognosis of gastric cancer patients.After transfection with Sam68 siRNA,cell proliferation,cell cycle progression,migration and invasion ability were decreased.Conclusions:Sam68 may be a new factor for the growth,migration and invasion of gastric cancer cells,and has significant value in predicting the prognosis of gastric cancer patients.
作者
高文
石永强
赵向阳
GAO Wen;SHI Yongqiang;ZHAO Xiangyang(Department of Gastroenterology, Nanjing Lishui People’s Hospital Zhongda Hospital Lishui Branch, Southeast University, Nanjing 211200)
出处
《胃肠病学》
北大核心
2021年第6期348-352,共5页
Chinese Journal of Gastroenterology
基金
南京医科大学康达学院科研发展基金(KD2018KYJJYB032)。
关键词
Sam68
胃肿瘤
预后
细胞增殖
细胞迁移
细胞侵袭
Sam68
Stomach Neoplasms
Prognosis
Cell Proliferation
Cell Migration
Cell Invasion
