摘要
目的研究附睾穿刺来源精子冷冻中添加褪黑素对精子冷冻效果的影响。方法收集2020-10/2021-03月到作者医院就诊的梗阻性无精子症男性行诊断性附睾穿刺来源精子62份,与患者沟通取得知情同意,检测精液的存活率、运动能力、DNA碎片指数(DNA fragmentation index, DFI)等指标。随机分为不添加额外保护剂的空白对照组和添加褪黑素的实验组,采用Vitro商品化精子冷冻液微量冷冻载杆冷冻,液氮保存24h后复苏,检测精液复苏后精子的各项指标,分析冷冻保护剂效果。结果冷冻复苏后实验组与对照组比较,精液的存活率无统计学差异;精子丙二醛(malondialdetioxidant, MDA)和细胞内活性氧(reactive oxygen species, ROS)较低,精子DFI较低,精子顶体完整性较高。结论附睾穿刺来源精子冷冻中添加褪黑素作为冷冻补充剂,对冷冻复苏附睾精子具有提高DNA和顶体完整性,降低氧化损伤水平的作用。
Objective To study the freezing effect of adding melatonin to frozen epididymal sperm.Methods A total of 62 samples of sperm from obstructive azoospermia men who were admitted to author′s hospital from October 2020 to March 2021 underwent diagnostic epididymal puncture were collected, the informed consent was obtained by communicating with the patients, and the survival rate of semen, exercise capacity, and DNA fragmentation index(DFI) were tested. These samples were randomly divided into a blank control group without additional protective agent and an experimental group with melatonin. All samples were frozen with Vitro commercial sperm freezing liquid micro-freezing rod, and were stored in liquid nitrogen for 24 hours and then recovered, and the sperm was detected after the recovery of the semen. The effects of cryoprotectants were analyzed.Results After freezing and resuscitation, the survival rate of semen between the experimental group and the control group was not statistically different;the sperm malondialdetioxidant(MDA) and intracellular reactive oxygen species(ROS) were lower, the sperm DFI was lower, and the sperm acrosome integrity was higher.Conclusion The addition of melatonin in the freezing of epididymal sperm as a freezing supplement has the effect of improving the integrity of DNA and acrosome, and reducing the level of oxidative damage to the frozen resuscitation of epididymal sperm.
作者
罗萌萌
张云霄
田蕊
赵铭佳
LUO Mengmeng;ZHANG Yunxiao;TIAN Rui;ZHAO Mingjia(Department of Reproductive Genetics,Maternal and Child Health Hospital of Tangshan City,Tangshan Hebei063000,China)
出处
《华南国防医学杂志》
CAS
2022年第1期16-19,共4页
Military Medical Journal of South China
基金
河北省医学科学研究课题(20191524)。
