摘要
目的 探究通过生物酶解技术有效得到羊乳高F值肽的关键工艺。方法 以新鲜山羊乳为原料,采用两种酶分步水解法制备高F值肽,以蛋白质水解度和F值为指标,通过单因素试验和响应面分析法分别确定两步酶解的最佳条件,酶解液经活性炭静态吸附去除游离芳香族氨基酸,对脱去芳香族氨基酸后的酶解液进行氨基酸组成分析并测定F值。结果 水解羊乳蛋白最佳内肽酶为碱性蛋白酶,最优工艺为:加酶量4000U/g、底物浓度6%、酶解pH 9.0、温度45℃、时间3 h;最佳外肽酶为风味蛋白酶,最优工艺为:加酶量6200 U/g、酶解pH 6.5、温度49℃、时间2 h。羊乳经两步酶解及活性炭吸附后, F值可达21.82,符合高F值肽的产品要求。结论 羊乳高F值肽的酶解工艺研究可为功能性营养食品的开发及陕西羊乳的高值化利用提供技术支撑。
Objective To explore the key process for effectively obtaining High Fischer Ratio Peptide from goat milk through biological enzymatic hydrolysis technology. Methods Using fresh goat milk as raw material, the High Fischer Ratio Peptide was prepared by a two-enzyme step-by-step hydrolysis method. Taking the degree of protein hydrolysis and F value as indicators, and the optimal conditions for the two-step enzymatic hydrolysis were determined by single factor and response surface analysis methods. Hydrolysates derived from goat milk protein were static adsorbed by activated carbon in order to remove liberated aromatic amino acids, the amino acid composition and F value of the hydrolysate after dearomatization were analyzed. Results Alkaline protease was selected as the best endopeptidase for hydrolyzing goat milk protein, the optimal processes were as follows: The ratio of enzyme to substrate 4000 U/g, substrate concentration 6%, pH 9.0, enzymatic temperature 45 ℃, and reaction time 3.0 h;flavourzyme was selected as the best exopeptidase enzyme, the optimal process was as follows: The ratio of enzyme to substrate 6200 U/g, enzymolysis pH 6.5, enzymatic temperature 49 ℃, and reaction time 2.0 h. After two-step enzymolysis of goat milk, the F value of the samples adsorbed by activated carbon reached 21.82, and met the product requirements of High Fischer Ratio Peptide. Conclusion The enzymatic hydrolysis process of goat milk High Fischer Ratio Peptide can provide technical support for the development of functional nutritional food and the high value utilization of Shaanxi goat milk.
作者
康鹏
葛武鹏
何锐
郝果
霍振泉
马宏祥
张闹
KANG Peng;GE Wu-Peng;HE Rui;HAO Guo;HUO Zhen-Quan;MA Hong-Xiang;ZHANG Nao(College of Food Science and Engineering,Northwest Agriculture&Forestry University,Yangling 712100,China;Shaanxi Baiyue Youlishi Dairy Industry Co.,Ltd.,Xianyang 712100,China;Shaanxi Goat Milk Product Quality Supervision and Inspection Center,Fuping 711700,China;Zhejiang Zhongmengchang Health Technology Co.,Ltd.,Hangzhou 310000,China;Shaanxi Hongxing Meiling Dairy Co.,Ltd.,Fuping 711700,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2022年第4期1240-1248,共9页
Journal of Food Safety and Quality
基金
陕西省重点研发计划项目(2021ZDLNY02-09)
校企合作项目(K4030220082)。
关键词
羊乳
酶解
高F值肽
工艺优化
goat milk
enzymolysis
High Fischer Ratio Peptide
process optimization
